The Clinical Use of Human Culture–Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects: A Pilot Study and Preliminary Results
Amgad M. Haleem,Abdel Aziz El Singergy,Dina Sabry,Hazem Atta,Laila A. Rashed,Constance R. Chu,Mohammed T. El Shewy,Akram Azzam,Mohammed Talaat Abdel Aziz +8 more
Reads0
Chats0
TLDR
Testing the hypothesis that platelet-rich fibrin glue can be used clinically as a scaffold to deliver autologous culture-expanded bone marrow mesenchymal stem cells (BM-MSCs) for cartilage repair and to report clinical results 1 y after implantation of MSCs PR-FG found it to be effective.Abstract:
Objective:To test the hypothesis that platelet-rich fibrin glue (PR-FG) can be used clinically as a scaffold to deliver autologous culture-expanded bone marrow mesenchymal stem cells (BM-MSCs) for cartilage repair and to report clinical results 1 y after implantation of MSCs PR-FG.Patients and Methods:Autologous BM-MSCs were culture expanded, placed on PR-FG intraoperatively, and then transplanted into 5 full-thickness cartilage defects of femoral condyles of 5 patients and covered with an autologous periosteal flap. Patients were evaluated clinically at 6 and 12 mo by the Lysholm and Revised Hospital for Special Surgery Knee (RHSSK) scores and radiographically by x-rays and magnetic resonance imaging (MRI) at the same time points. Repair tissue in 2 patients was rated arthroscopically after 12 mo using the International Cartilage Repair Society (ICRS) Arthroscopic Score.Study Design:Case series; level of evidence 4.Results:All patients’ symptoms improved over the follow-up period of 12 mo. Average Lyshol...read more
Citations
More filters
Journal ArticleDOI
Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine
TL;DR: Allogeneic MSC treatments, categorized as a drug by regulatory agencies, have been widely pursued, but new studies demonstrate the efficacy of autologous MSC therapies, even for individuals affected by a disease state.
Journal ArticleDOI
Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche.
TL;DR: A synergistic relationship between osteocytes and osteoblasts in producing biochemical signals to stimulate the osteogenic differentiation of MSCs is confirmed and a possible role for the use of co-culture or conditioned media methodologies for tissue engineering applications is outlined.
Journal ArticleDOI
Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis: a review
TL;DR: It is unlikely that a mix of GFs some of which have negative effects in the OA joint, as present in PRP, will be of benefit in OA, so future directions of PRP application may concentrate on seeking an appropriate and innocuous agent like anti-VEGF antibody that can modulate and control the effect ofPRP.
Journal ArticleDOI
Mesenchymal stem cells for cartilage repair in osteoarthritis
TL;DR: By secreting various bioactive soluble factors, BMSCs can protect the cartilage from further tissue destruction and facilitate regeneration of the remaining progenitor cells in situ and their therapeutic potential for repairing cartilage damage in OA is described.
Journal ArticleDOI
Biology of platelet-rich plasma and its clinical application in cartilage repair
TL;DR: Clinical trials suggest that PRP may have the potential to fill cartilage defects to enhance cartilage repair, attenuate symptoms of osteoarthritis and improve joint function, with an acceptable safety profile.
References
More filters
Journal ArticleDOI
Platelet-rich plasma: evidence to support its use
TL;DR: Not all currently marketed PRP devices are qual; some do not concentrate viably active platelets n sufficient numbers to produce a healing enhance- herefore, the term PRP is preferred to autologous latelet gel, plasma-rich growth factors (PRGFs), or a ere autOLOGous platelet concentrate.
Journal ArticleDOI
Mesenchymal Cell-Based Repair of Large Full Thickness Defects of Articular Cartilage
Shigeyuki Wakitani,Tatsuhiko Goto,S. Pineda,Randell G. Young,Joseph M. Mansour,Arnold I. Caplan,Victor M. Goldberg +6 more
TL;DR: Osteochondral progenitor cells were used to repair large, full-thickness defects of the articular cartilage that had been created in the knees of rabbits.
Journal ArticleDOI
Chondrogenic Differentiation of Cultured Human Mesenchymal Stem Cells from Marrow
Alastair Morgan Mackay,Stephen C. Beck,J. Mary Murphy,Frank Barry,Clinton O. Chichester,Mark F. Pittenger +5 more
TL;DR: Increased understanding of the induction of chondrogenic differentiation should lead to further progress in defining the mechanisms responsible for the generation of cartilaginous tissues, their maintenance, and their regeneration.
Journal Article
Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation.
TL;DR: The long-term follow-up of small series of patients has shown that the transplantation of osteochondral autologous grafts and allografts can be effective for the treatment of focal defects of articular cartilage in selected patients.
Journal ArticleDOI
Why are MSCs therapeutic? New data: new insight
TL;DR: A new, enlightened era of experimentation and clinical trials has been initiated with xenogenic and allogeneic MSCs.