Journal ArticleDOI
The efficiency of systematic sampling in stereology and its prediction
TLDR
A set of very simple estimators of efficiency are presented and illustrated with a variety of biological examples and a nomogram for predicting the necessary number of points when performing point counting is provided.Abstract:
The superior efficiency of systematic sampling at all levels in stereological studies is emphasized and various commonly used ways of implementing it are briefly described. Summarizing recent theoretical and experimental studies a set of very simple estimators of efficiency are presented and illustrated with a variety of biological examples. In particular, a nomogram for predicting the necessary number of points when performing point counting is provided. The very efficient and simple unbiased estimator of the volume of an arbitrary object based on Cavalieri's principle is dealt with in some detail. The efficiency of the systematic fractionating of an object is also illustrated.read more
Citations
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Journal ArticleDOI
Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator
TL;DR: A stereological method for obtaining estimates of the total number of neurons in five major subdivisions of the rat hippocampus is described, and a number of new stereological methods that have particular relevance to the quantitative study of the structure of the nervous system are briefly described in an appendix.
Journal ArticleDOI
Some new, simple and efficient stereological methods and their use in pathological research and diagnosis.
Hj. Gundersen,Tf. Bendtsen,Lise Korbo,Niels Marcussen,Arne Møller,K. Nielsen,Jr. Nyengaard,Bente Pakkenberg,Flemming Brandt Sørensen,A. Vesterby +9 more
TL;DR: Methods for estimating the volume, surface area and length of any structure are described in this review and the principles on which stereology is based and the necessary sampling procedures are described and illustrated with examples.
Journal ArticleDOI
The new stereological tools: disector, fractionator, nucleator and point-sampled intercepts and their use in pathological research and diagnosis
H. J. G. Gundersen,P. V. Bagger,Thomas Fichtner Bendtsen,S.M. Evans,L. Korbo,Niels Marcussen,Arne Møller,Karsten Nielsen,Jens R. Nyengaard,Bente Pakkenberg,Sørensen Fb,A. Vesterby,Mark J. West +12 more
TL;DR: The new stereological methods for correct and efficient sampling and sizing of cells and other particles are reviewed and practical examples of applications to a wide range of histological entities are illustrated.
Journal ArticleDOI
Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects.
TL;DR: The existence of many new and encouraging biological approaches to cartilage repair justifies the future investment of time and money in this research area, particularly given the extremely high socio-economic importance of such therapeutic strategies in the prevention and treatment of these common joint diseases and traumas.
Journal ArticleDOI
Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson
TL;DR: The full range of estimators is described, some of them for the first time, some in an improved form, several in more than one version, and all of them under the single, absolute requirement that one can in fact identify what one is quantifying on sections.
References
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Journal ArticleDOI
The unbiased estimation of number and sizes of arbitrary particles using the disector
TL;DR: A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.
Journal ArticleDOI
Practical stereological methods for morphometric cytology.
TL;DR: Some principles which allow the estimation of volumetric ratios, surface areas, surface-to-volume ratios, thicknesses of tissue or cell sheets, and the number of structures are reviewed and presented in general form.
Journal ArticleDOI
Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson
TL;DR: The full range of estimators is described, some of them for the first time, some in an improved form, several in more than one version, and all of them under the single, absolute requirement that one can in fact identify what one is quantifying on sections.
Journal ArticleDOI
Estimation of surface area from vertical sections.
TL;DR: ‘Vertical’ sections are plane sections longitudinal to a fixed (but arbitrary) axial direction that can be generated by placing the object on a table and taking sections perpendicular to the plane of the table.