The unbiased estimation of number and sizes of arbitrary particles using the disector
TLDR
A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.Abstract:
A three-dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented. Used in combination with ordinary and recently developed stereological methods unbiased estimates of various mean particle sizes and the variance of particle volume are obtainable on sets of two parallel sections with a known separation. The same principle allows the unbiased estimation of the distribution of individual particle volumes in sets of serial sections.read more
Citations
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Journal ArticleDOI
The efficiency of systematic sampling in stereology and its prediction
TL;DR: A set of very simple estimators of efficiency are presented and illustrated with a variety of biological examples and a nomogram for predicting the necessary number of points when performing point counting is provided.
Journal ArticleDOI
Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator
TL;DR: A stereological method for obtaining estimates of the total number of neurons in five major subdivisions of the rat hippocampus is described, and a number of new stereological methods that have particular relevance to the quantitative study of the structure of the nervous system are briefly described in an appendix.
Journal ArticleDOI
The new stereological tools: disector, fractionator, nucleator and point-sampled intercepts and their use in pathological research and diagnosis
H. J. G. Gundersen,P. V. Bagger,Thomas Fichtner Bendtsen,S.M. Evans,L. Korbo,Niels Marcussen,Arne Møller,Karsten Nielsen,Jens R. Nyengaard,Bente Pakkenberg,Sørensen Fb,A. Vesterby,Mark J. West +12 more
TL;DR: The new stereological methods for correct and efficient sampling and sizing of cells and other particles are reviewed and practical examples of applications to a wide range of histological entities are illustrated.
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Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson
TL;DR: The full range of estimators is described, some of them for the first time, some in an improved form, several in more than one version, and all of them under the single, absolute requirement that one can in fact identify what one is quantifying on sections.
Journal ArticleDOI
Life and Death of Neurons in the Aging Brain
John H. Morrison,Patrick R. Hof +1 more
TL;DR: The qualitative and quantitative differences between aging and Alzheimer's disease with respect to neuron loss are discussed, and age-related changes in functional and biochemical attributes of hippocampal circuits that might mediate functional decline in the absence of neuron death are explored.
References
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Journal ArticleDOI
Notes on the estimation of the numerical density of arbitrary profiles: the edge effect
TL;DR: In this paper, a family of test-frames for obtaining an unbiased estimate of the numerical density of arbitrary profiles on a section is described, and the counting rule pertaining to the test-frame is simple and requires no corrections based on other estimated quantities.
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Optimizing sampling efficiency of stereological studies in biology: or 'do more less well!'.
H. J. G. Gundersen,R. ØSterby +1 more
TL;DR: The aim of the sampling design for stereology is to obtain the maximal amount of quantitative structural information at a given total cost or effort, which is irrational in almost all studies where the emphasis is on the biological results.
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Precise and general conditions for the validity of a comprehensive set of stereological fundamental formulae
R. E. Miles,Pamela Davy +1 more
TL;DR: In this article, the mean lineal and areal projections of embedded features are estimated using unbiased ratio estimators of stereological fractions for various types of random plane and line sections of the specimen.