Journal ArticleDOI
The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution*
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TLDR
In this paper, specific absorption coefficients for individual carotenoids and chlorophylls a and b, as well as the E 1% 1cm values for combined carotensoids, have been (re)estimated using 6 solvents (80 % acetone, chloroform, diethyl ether, dimethyl formamide and methanol) using two different types of spectrophotometer (0.1-0.5 nm and 1-4 nm band pass resolution).About:
This article is published in Journal of Plant Physiology.The article was published on 1994-09-01. It has received 4156 citations till now. The article focuses on the topics: Chlorophyll b.read more
Citations
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Journal ArticleDOI
The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b
TL;DR: How the ratios are increasingly and alarmingly low as the proportion of chlorophyll a increases is described, which is always low.
Journal ArticleDOI
Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light
Sander W. Hogewoning,G. Trouwborst,Hans Maljaars,Hendrik Poorter,Wim van Ieperen,Jeremy Harbinson +5 more
TL;DR: It is concluded that blue light during growth is qualitatively required for normal photosynthetic functioning and quantitatively mediates leaf responses resembling those to irradiance intensity.
Journal ArticleDOI
Assessing vineyard condition with hyperspectral indices: Leaf and canopy reflectance simulation in a row-structured discontinuous canopy
Pablo J. Zarco-Tejada,Alberto Berjón,Raúl López-Lozano,John R. Miller,Pedro Martín,Victoria E. Cachorro,M. R. González,A. M. de Frutos +7 more
TL;DR: In this article, a Li-Cor 1800-12 Integrating Sphere coupled with a 200 Am diameter single mode fiber to an Ocean Optics model USB2000 spectrometer was used for measuring the optical properties of reflectance and transmittance with a subsample of 605 leaves.
Journal ArticleDOI
Why Leaves Turn Red in Autumn. The Role of Anthocyanins in Senescing Leaves of Red-Osier Dogwood
TL;DR: Evidence is provided for red-osier dogwood that anthocyanins form a pigment layer in the palisade mesophyll layer that decreases light capture by chloroplasts that reduces risk of photo-oxidative damage to leaf cells as they senesce, which otherwise may lower the efficiency of nutrient retrieval from senescing autumn leaves.
Journal ArticleDOI
Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves
TL;DR: The SPAD-502 has been shown to be a good tool to diagnose the integrity of the photosynthetic system in coffee leaves, and can thus help in the advanced interpretations of the photochemical process of these plants.
References
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Journal ArticleDOI
Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
TL;DR: Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
Book ChapterDOI
Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes
TL;DR: In this article, the spectral characteristics and absorption coefficients of chlorophylls, pheophytins, and carotenoids were analyzed using a two-beam spectrophotometer.
Journal ArticleDOI
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
TL;DR: In this paper, the extinction coefficients for chlorophylls a and b in diethylether (Smith, J.H. and Benitez, A.V., eds.), used in this paper as primary standards, were verified by magnesium determination using atomic absorbance spectrophotometry.
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Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents
TL;DR: Pfister et al. as mentioned in this paper used a t.1.c. method (Lichtenthaler 8t Pfister, 1978) that permits a distinct separation of the two chlorophylls and also the major carotenoids using light petroleum (b.p. 40-6O0C)/dioxane/propan-2-ol (7 :3 : 1, by vol.) as a developing solvent.
Journal ArticleDOI
Absorption of light by chlorophyll solutions
TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.