Journal ArticleDOI
Rapid determination of free proline for water-stress studies
TLDR
In this article, a simple colorimetric determination of proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material was presented.Abstract:
Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.read more
Citations
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Journal ArticleDOI
The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat
TL;DR: The physiological and biochemical parameters measured indicate the UV-B light has stronger stress effectors than drought on the growth of seedlings of both species.
Journal ArticleDOI
Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants
TL;DR: Proline (Pro) accumulation has been correlated with tolerance to drought and salinity stresses in plants and overproduction of Pro in plants may lead to increased tolerance against these abiotic stresses, suggesting that activity of the first enzyme of the pathway is the rate-limiting factor in Pro synthesis.
Journal ArticleDOI
Implications of water stress‐induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings
S. P. Mukherjee,M. A. Choudhuri +1 more
TL;DR: In this article, the authors show that treatment with antioxidants makes the plant tolerant against water stress by modulating the endogenous levels of H2O2 and ascorbic acid in stressed tissue.
Journal ArticleDOI
Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit
TL;DR: The results demonstrate that with a combination of breeding and transgenic plants it could be possible to produce salt-tolerant crops with far fewer target traits than had been anticipated and the utility of such a modification in preserving the quality of the fruit.
Journal ArticleDOI
Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration
TL;DR: Results show that salinity tolerance of Kharchia 65 as manifested by lower decrease in biomass and grain yield is associated with higher antioxidant activity, osmolyte concentration and potassium contents, and lower H2O2, TBARS and sodium contents than KRL 19.
References
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Journal ArticleDOI
A photometric method for the determination of proline.
Walter Troll,John Lindsley +1 more
TL;DR: In the present method, a modification of the procedure of Chinard (l), the interfering basic amino acids lysine, hydroxylysine, and ornithine are removed by shaking the solutions with Permutit and the method becomes entirely specific for proline when applied to protein hydrolysates, urine, or plasma.
Journal ArticleDOI
Amino Acid and Protein Metabolism in Bermuda Grass During Water Stress
N. M. Barnett,Aubrey W. Naylor +1 more
TL;DR: No significant differences were found in the amino acid and protein metabolism of the 2 varieties of Bermuda grass, and it is suggested that during water stress free proline functions as a storage compound.
Journal ArticleDOI
Changes in Amino Acid Content of Excised Leaves During Incubation I. The Effect of Water Content of Leaves and Atmospheric Oxygen Level
TL;DR: The amino acid analyses showed that turgid leaves did not accumulate prolines and that proline and methylcysteine sulfoxide accumulation was abolished by anaerobiosis, and several pathways of amino acid degradation were suggested.
Journal ArticleDOI
An improved colorimetric method for the determination of proline in the presence of other ninhydrin-positive compounds.
J. J. Wren,P. H. Wiggall +1 more
TL;DR: A method applicable to protein samples was developed and the only compound found to interfere appreciably was a hydroxyproline.