Reactive oxygen species (ROS) were initially recognized as toxic by-products of aerobic metabolism. In recent years, it has become apparent that ROS plays an important signaling role in plants, controlling processes such as growth, development and especially response to biotic and abiotic environmental stimuli. The major members of the ROS family include free radicals like O2● −, OH● and non-radicals like H2O2 and 1O2. The ROS production in plants is mainly localized in the chloroplast, mitochondria and peroxisomes. There are secondary sites as well like the endoplasmic reticulum, cell membrane, cell wall and the apoplast. The role of the ROS family is that of a double edged sword; while they act as secondary messengers in various key physiological phenomena, they also induce oxidative damages under several environmental stress conditions like salinity, drought, cold, heavy metals, UV irradiation etc., when the delicate balance between ROS production and elimination, necessary for normal cellular homeostasis, is disturbed. The cellular damages are manifested in the form of degradation of biomolecules like pigments, proteins, lipids, carbohydrates and DNA, which ultimately amalgamate in plant cellular death. To ensure survival, plants have developed efficient antioxidant machinery having two arms, (i) enzymatic components like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR); (ii) non-enzymatic antioxidants like ascorbic acid (AA), reduced glutathione (GSH), α-tocopherol, carotenoids, flavonoids and the osmolyte proline. These two components work hand in hand to scavenge ROS. In this review, we emphasize on the different types of ROS, their cellular production sites, their targets, and their scavenging mechanism mediated by both the branches of the antioxidant systems, highlighting the potential role of antioxidant

Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants

Reactive oxygen species

Salinity is a major abiotic stress limiting growth and productivity of plants in many areas of the world due to increasing use of poor quality of water for irrigation and soil salinization. Plant adaptation or tolerance to salinity stress involves complex physiological traits, metabolic pathways, and molecular or gene networks. A comprehensive understanding on how plants respond to salinity stress at different levels and an integrated approach of combining molecular tools with physiological and biochemical techniques are imperative for the development of salt-tolerant varieties of plants in salt-affected areas. Recent research has identified various adaptive responses to salinity stress at molecular, cellular, metabolic, and physiological levels, although mechanisms underlying salinity tolerance are far from being completely understood. This paper provides a comprehensive review of major research advances on biochemical, physiological, and molecular mechanisms regulating plant adaptation and tolerance to salinity stress.

/pdf/mechanism-of-salinity-tolerance-in-plants-physiological-3ixvqhezre.pdf

Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization

High temperature (HT) stress is a major environmental stress that limits plant growth, metabolism, and productivity worldwide Plant growth and development involve numerous biochemical reactions that are sensitive to temperature Plant responses to HT vary with the degree and duration of HT and the plant type HT is now a major concern for crop production and approaches for sustaining high yields of crop plants under HT stress are important agricultural goals Plants possess a number of adaptive, avoidance, or acclimation mechanisms to cope with HT situations In addition, major tolerance mechanisms that employ ion transporters, proteins, osmoprotectants, antioxidants, and other factors involved in signaling cascades and transcriptional control are activated to offset stress-induced biochemical and physiological alterations Plant survival under HT stress depends on the ability to perceive the HT stimulus, generate and transmit the signal, and initiate appropriate physiological and biochemical changes HT-induced gene expression and metabolite synthesis also substantially improve tolerance The physiological and biochemical responses to heat stress are active research areas, and the molecular approaches are being adopted for developing HT tolerance in plants This article reviews the recent findings on responses, adaptation, and tolerance to HT at the cellular, organellar, and whole plant levels and describes various approaches being taken to enhance thermotolerance in plants

Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants


 The spectral ratio between horizontal and vertical components (H/V ratio) of microtremors measured at the ground surface has been used to estimate fundamental periods and amplification factors of a site, although this technique lacks theoretical background. The aim of this article is to formulate the H/V technique in terms of the characteristics of Rayleigh and Love waves, and to contribute to improve the technique. The improvement includes use of not only peaks but also troughs in the H/V ratio for reliable estimation of the period and use of a newly proposed smoothing function for better estimation of the amplification factor. The formulation leads to a simple formula for the amplification factor expressed with the H/V ratio. With microtremor data measured at 546 junior high schools in 23 wards of Tokyo, the improved technique is applied to mapping site periods and amplification factors in the area.

Ground Motion Characteristics Estimated from Spectral Ratio between Horizontal and Verticcl Components of Mietremors.

Reactive oxygen species (ROS) generation is a usual phenomenon in a plant both under a normal and stressed condition. However, under unfavorable or adverse conditions, ROS production exceeds the capacity of the antioxidant defense system. Both non-enzymatic and enzymatic components of the antioxidant defense system either detoxify or scavenge ROS and mitigate their deleterious effects. The Ascorbate-Glutathione (AsA-GSH) pathway, also known as Asada–Halliwell pathway comprises of AsA, GSH, and four enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, play a vital role in detoxifying ROS. Apart from ROS detoxification, they also interact with other defense systems in plants and protect the plants from various abiotic stress-induced damages. Several plant studies revealed that the upregulation or overexpression of AsA-GSH pathway enzymes and the enhancement of the AsA and GSH levels conferred plants better tolerance to abiotic stresses by reducing the ROS. In this review, we summarize the recent progress of the research on AsA-GSH pathway in terms of oxidative stress tolerance in plants. We also focus on the defense mechanisms as well as molecular interactions.

https://www.mdpi.com/2076-3921/8/9/384/pdf

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Glutathione (GSH; γ-glutamyl-cysteinyl-glycine) is a small intracellular thiol molecule which is considered as a strong non-enzymatic antioxidant. Glutathione regulates multiple metabolic functions; for example, it protects membranes by maintaining the reduced state of both α-tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S-transferase. By acting as a precursor of phytochelatins, GSH helps in the chelating of toxic metals/metalloids which are then transported and sequestered in the vacuole. The glyoxalase pathway (consisting of glyoxalase I and glyoxalase II enzymes) for detoxification of methylglyoxal, a cytotoxic molecule, also requires GSH in the first reaction step. For these reasons, much attention has recently been directed to elucidation of the role of this molecule in conferring tolerance to abiotic stress. Recently, this molecule has drawn much attention because of its interaction with other signaling molecules and phytohormones. In this review, we have discussed the recent progress in GSH biosynthesis, metabolism and its role in abiotic stress tolerance.

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Among the plant nutrients, potassium (K) is one of the vital elements required for plant growth and physiology. Potassium is not only a constituent of the plant structure but it also has a regulatory function in several biochemical processes related to protein synthesis, carbohydrate metabolism, and enzyme activation. Several physiological processes depend on K, such as stomatal regulation and photosynthesis. In recent decades, K was found to provide abiotic stress tolerance. Under salt stress, K helps to maintain ion homeostasis and to regulate the osmotic balance. Under drought stress conditions, K regulates stomatal opening and helps plants adapt to water deficits. Many reports support the notion that K enhances antioxidant defense in plants and therefore protects them from oxidative stress under various environmental adversities. In addition, this element provides some cellular signaling alone or in association with other signaling molecules and phytohormones. Although considerable progress has been made in understanding K-induced abiotic stress tolerance in plants, the exact molecular mechanisms of these protections are still under investigation. In this review, we summarized the recent literature on the biological functions of K, its uptake, its translocation, and its role in plant abiotic stress tolerance.

Potassium: A Vital Regulator of Plant Responses and Tolerance to Abiotic Stresses

Plants are frequently exposed to a plethora of unfavorable or even adverse environmental conditions, termed as abiotic stresses (such as salinity, drought, heat, cold, flooding, heavy metals, ozone, UV radiation, etc.) and thus they pose serious threats to the sustainability of crop yield. Soil salinity, one of the most severe abiotic stresses, limits the production of about 6 % of the world’s total land and 20 % of irrigated land (17 % of total cultivated areas) and negatively affects crop production worldwide. On the other hand, increased salinity of agricultural land is expected to have destructive global effects, resulting in up to 50 % land loss by the next couple of decades. The adverse effects of salinity have been ascribed mainly to an increase in sodium (Na+) and chloride (Cl–) ions and hence these ions produce the critical conditions for plant survival by intercepting different plant mechanisms. Both Na+ and Cl– produce many physiological disorders in plants but Cl– is the most dangerous. A plant’s response to salt stress depends on the genotype, developmental stage, as well as the intensity and duration of the stress. Increased salinity has diverse effects on the physiology of plants grown in saline conditions and in response to major factors like osmotic stress, ion-specificity, nutritional and hormonal imbalance, and oxidative damage. In addition to upper plant parts, salinity also affects root growth and physiology and their function in nutrient uptake. The outcome of these effects may cause the disorganization of cellular membranes, inhibit photosynthesis, generate toxic metabolites and decline nutrient absorption, ultimately leading to plant death. In recent decades, exogenous protectants such as osmoprotectants, phytohormones, signaling molecules, polyamines, antioxidants and various trace elements have been found effective in plants in mitigating the salt induced damages. These protectants showed the capacity to enhance the plants’ growth, yield as well as stress tolerance under salinity. In this chapter we attempt to summarize differential responses of plants to salinity with special reference to growth, physiology and yield. Further, we have discussed the progress made in using exogenous protectants to mitigate salt-induced damages in plants.

https://www.springer.com/cda/content/document/cda_downloaddocument/9781461447467-c1.pdf

Kamrun Nahar

Papers

Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Potassium: A Vital Regulator of Plant Responses and Tolerance to Abiotic Stresses

Plant Response to Salt Stress and Role of Exogenous Protectants to Mitigate Salt-Induced Damages