Michael E. Lee

TL;DR: By extending guide RNAs to include effector protein recruitment sites, this work constructs modular scaffold RNAs that encode both target locus and regulatory action and applies this approach to flexibly redirect flux through a complex branched metabolic pathway in yeast.

TL;DR: A versatile engineering platform for yeast, which contains both a rapid, modular assembly method and a basic set of characterized parts, and genome-editing tools for making modifications directly to the yeast chromosomes, which the authors find preferable to plasmids due to reduced variability in expression.

TL;DR: Mutations recovered in the best cellodextrin transporters reveal synergy between substrate binding and transporter dynamics, and demonstrate the power of CRISPRm to accelerate selection experiments and discoveries of the molecular determinants that enhance biomolecule function.

TL;DR: This work characterized a set of constitutive promoters in Saccharomyces cerevisiae that spanned a wide range of expression and maintained their relative strengths irrespective of the coding sequence and trained a regression model on a random sample comprising 3% of the total library, and used that model to predict genotypes that would preferentially produce each of the products in this highly branched pathway.

TL;DR: Differences in promoter enrichment for the three-gene library depending on whether the pentose phosphate pathway genes were included during the aerobic enrichment are observed and the importance of selection conditions is observed.