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William C. DeLoache
Researcher at University of California, Berkeley
Publications - 19
Citations - 2116
William C. DeLoache is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 12, co-authored 19 publications receiving 1675 citations. Previous affiliations of William C. DeLoache include Stanford University & Davidson College.
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Journal ArticleDOI
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
TL;DR: A versatile engineering platform for yeast, which contains both a rapid, modular assembly method and a basic set of characterized parts, and genome-editing tools for making modifications directly to the yeast chromosomes, which the authors find preferable to plasmids due to reduced variability in expression.
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Selection of chromosomal DNA libraries using a multiplex CRISPR system
Owen Ryan,Jeffrey M. Skerker,Matthew J. Maurer,Xin Li,Jordan C. Tsai,Snigdha Poddar,Michael E. Lee,William C. DeLoache,John E. Dueber,Adam P. Arkin,Jamie H. D. Cate +10 more
TL;DR: Mutations recovered in the best cellodextrin transporters reveal synergy between substrate binding and transporter dynamics, and demonstrate the power of CRISPRm to accelerate selection experiments and discoveries of the molecular determinants that enhance biomolecule function.
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An enzyme-coupled biosensor enables ( S )-reticuline production in yeast from glucose
William C. DeLoache,Zachary N. Russ,Lauren Narcross,Andrew M Gonzales,Vincent J. J. Martin,John E. Dueber +5 more
TL;DR: This work demonstrates the production of the key BIA intermediate (S)-reticuline from glucose in Saccharomyces cerevisiae and develops an enzyme-coupled biosensor for the upstream intermediate L-3,4-dihydroxyphenylalanine (L-DOPA).
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Spatial organization of enzymes for metabolic engineering.
TL;DR: A critical challenge for achieving ideal pathway compartmentalization with protein shells will likely be evolving pores to selectively limit intermediate diffusion, which should enhance the ability to rationally design metabolic pathways.
Journal ArticleDOI
An exclusive metabolic niche enables strain engraftment in the gut microbiota.
Elizabeth Stanley Shepherd,William C. DeLoache,Kali M. Pruss,Weston R. Whitaker,Justin L. Sonnenburg +4 more
TL;DR: Finely tuned control of strain engraftment and abundance in the mouse gut microbiota was achieved using the marine polysaccharide porphyran, which could exclusively be used by an introduced subset of wild-type or genetically modified Bacteroides strains.