R
Ryan E. Cobb
Researcher at University of Illinois at Urbana–Champaign
Publications - 16
Citations - 1703
Ryan E. Cobb is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Genome editing & Synthetic biology. The author has an hindex of 12, co-authored 16 publications receiving 1439 citations. Previous affiliations of Ryan E. Cobb include Urbana University.
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High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system.
TL;DR: The designed pCRISPomyces plasmids are amenable to assembly of spacers and editing templates via Golden Gate assembly and isothermal assembly, respectively, allowing rapid plasmid construction to target any genomic locus of interest.
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CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Mingzi M. Zhang,Fong Tian Wong,Yajie Wang,Shangwen Luo,Yee Hwee Lim,Elena Heng,Wan Lin Yeo,Ryan E. Cobb,Behnam Enghiad,Ee Lui Ang,Huimin Zhao,Huimin Zhao +11 more
TL;DR: An efficient CRISPR-Cas9 knock-in strategy is reported to activate silent biosynthetic gene clusters (BGCs) in streptomycetes and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes.
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Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster
TL;DR: A plug and play synthetic biology strategy is applied to activate a cryptic PTM biosynthetic gene cluster SGR810-815 from Streptomyces griseus and discover three potential PTMs which resembles iterative PKSs known in fungi.
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CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
TL;DR: High expression levels of the Cas9 protein in Synechococcus 2973 appear to be toxic and result in cell death, but introduction of a CRISPR/Cas9 genome editing system on a plasmid backbone that leads to transient cas9 expression allowed for efficient markerless genome editing in a wild type genetic background.
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Directed Evolution: Past, Present, and Future
TL;DR: Some of the major milestones in the history of directed evolution are recounted, the most promising recent developments in the field are highlighted, and the future challenges and opportunities that lie ahead are discussed.