H
Huimin Zhao
Researcher at University of Illinois at Urbana–Champaign
Publications - 463
Citations - 25116
Huimin Zhao is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Synthetic biology & Directed evolution. The author has an hindex of 78, co-authored 428 publications receiving 20651 citations. Previous affiliations of Huimin Zhao include California Institute of Technology & Dow Chemical Company.
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Journal ArticleDOI
Chromatin architecture reorganization during stem cell differentiation
Jesse R. Dixon,Inkyung Jung,Siddarth Selvaraj,Yin Shen,Jessica Antosiewicz-Bourget,Ah Young Lee,Zhen Ye,Audrey Kim,Nisha Rajagopal,Wei Xie,Yarui Diao,Jing Liang,Huimin Zhao,Victor V. Lobanenkov,Joseph R. Ecker,James A. Thomson,Bing Ren +16 more
TL;DR: Mapping genome-wide chromatin interactions in human embryonic stem cells and four human ES-cell-derived lineages reveals extensive chromatin reorganization during lineage specification, providing a global view of chromatin dynamics and a resource for studying long-range control of gene expression in distinct human cell lineages.
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Molecular evolution by staggered extension process (StEP) in vitro recombination
TL;DR: The staggered extension process (StEP) consists of priming the template sequence(s) followed by repeated cycles of denaturation and extremely abbreviated annealing/polymerase-catalyzed extension, which results in recombination of polynu-cleotide sequences.
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DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
Zengyi Shao,Hua Zhao,Huimin Zhao +2 more
TL;DR: A new method, DNA assembler, is reported, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae and represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.
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High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system.
TL;DR: The designed pCRISPomyces plasmids are amenable to assembly of spacers and editing templates via Golden Gate assembly and isothermal assembly, respectively, allowing rapid plasmid construction to target any genomic locus of interest.
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Recent developments in pyridine nucleotide regeneration.
TL;DR: The use of electrochemical methods has allowed cofactor regeneration for monooxygenases and natural or engineered whole-cell systems provide alternatives to approaches relying on purified enzymes.