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Yajie Wang
Researcher at University of Illinois at Urbana–Champaign
Publications - 73
Citations - 2500
Yajie Wang is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 19, co-authored 47 publications receiving 1739 citations. Previous affiliations of Yajie Wang include Harbin Institute of Technology & Wuhan University.
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High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system.
TL;DR: The designed pCRISPomyces plasmids are amenable to assembly of spacers and editing templates via Golden Gate assembly and isothermal assembly, respectively, allowing rapid plasmid construction to target any genomic locus of interest.
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Microbial alginate production, modification and its applications
TL;DR: Alginate is an important polysaccharide used widely in the food, textile, printing and pharmaceutical industries for its viscosifying, and gelling properties and the potential to utilize these bacterially produced or modified alginates for high‐value applications where defined material properties are required.
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CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Mingzi M. Zhang,Fong Tian Wong,Yajie Wang,Shangwen Luo,Yee Hwee Lim,Elena Heng,Wan Lin Yeo,Ryan E. Cobb,Behnam Enghiad,Ee Lui Ang,Huimin Zhao,Huimin Zhao +11 more
TL;DR: An efficient CRISPR-Cas9 knock-in strategy is reported to activate silent biosynthetic gene clusters (BGCs) in streptomycetes and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes.
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Cooperative asymmetric reactions combining photocatalysis and enzymatic catalysis
TL;DR: A class of cooperative chemoenzymatic reaction that combines photocatalysts that isomerize alkenes with ene-reductases that reduce carbon–carbon double bonds to generate valuable enantioenriched products and provides a general strategy for converting stereoselective enzymatic reactions into stereoconvergent ones.
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Directed Evolution: Methodologies and Applications.
TL;DR: Directed evolution aims to expedite the natural evolution process of biological molecules and systems in a test tube through iterative rounds of gene diversifications and library screening/selection.