Satoshi Yamamoto

TL;DR: New ITS primers with improved coverage across diverse taxonomic groups of fungi compared to existing primers are designed to provide a basis for ecological studies on the diversity and community structures of fungi in the era of massive DNA sequencing.

TL;DR: The metabarcoding approach presented here is non-invasive, more efficient, more cost-effective and more sensitive than the traditional survey methods and has the potential to serve as an alternative tool for biodiversity monitoring that revolutionizes natural resource management and ecological studies of fish communities on larger spatial and temporal scales.

TL;DR: The ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters by using high-performance fish-universal primers and systematic spatial water sampling at 47 stations covering ~11 km2 revealed the fish community structure at a species resolution.

TL;DR: It is concluded that eDNA generally provides a ‘snapshot’ of fish distribution and biomass in a large area and a best-fit model included echo intensity obtained within 10–150 m from water sampling sites, indicating that the estimated eDNA concentration most likely reflects fish biomass within 150 m in the bay.

TL;DR: It was shown that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass and that the small‐sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass.