MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species.
Masaki Miya,Yukuto Sato,Tsukasa Fukunaga,Tetsuya Sado,J. Y. Poulsen,Keiichi Sato,Toshifumi Minamoto,Satoshi Yamamoto,Hiroki Yamanaka,Hitoshi Araki,Michio Kondoh,Wataru Iwasaki +11 more
TLDR
The metabarcoding approach presented here is non-invasive, more efficient, more cost-effective and more sensitive than the traditional survey methods and has the potential to serve as an alternative tool for biodiversity monitoring that revolutionizes natural resource management and ecological studies of fish communities on larger spatial and temporal scales.Abstract:
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequence...read more
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Environmental DNA metabarcoding: transforming how we survey animal and plant communities
Kristy Deiner,Holly M. Bik,Elvira Mächler,Elvira Mächler,Mathew Seymour,Anaïs Lacoursière-Roussel,Florian Altermatt,Florian Altermatt,Simon Creer,Iliana Bista,Iliana Bista,David M. Lodge,Natasha de Vere,Michael E. Pfrender,Louis Bernatchez +14 more
TL;DR: The use of eDNA metabarcoding for surveying animal and plant richness, and the challenges in using eDNA approaches to estimate relative abundance are reviewed, which distill what is known about the ability of different eDNA sample types to approximate richness in space and across time.
Journal ArticleDOI
Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
Alice Valentini,Pierre Taberlet,Pierre Taberlet,Claude Miaud,Raphaël Civade,Jelger Herder,Philip Francis Thomsen,Eva Bellemain,Aurélien Besnard,Eric Coissac,Eric Coissac,Frédéric Boyer,Frédéric Boyer,Coline Gaboriaud,Pauline Jean,Nicolas Poulet,Nicolas Roset,Gordon H. Copp,Gordon H. Copp,Philippe Geniez,Didier Pont,Christine Argillier,Jean-Marc Baudoin,Tiphaine Peroux,Alain J. Crivelli,Anthony Olivier,Manon Acqueberge,Matthieu Le Brun,Peter Rask Møller,Eske Willerslev,Tony Dejean +30 more
TL;DR: For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI = 0.90-0.99) vs. 0.58 (CI=0.50-0.63) for traditional surveys as mentioned in this paper.
Data from: Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
Alice Valentini,Pierre Taberlet,Claude Miaud,Raphaël Civade,Jelger Herder,Philip Francis Thomsen,Eva Bellemain,Aurélien Besnard,Eric Coissac,Frédéric Boyer,Coline Gaboriaud,Pauline Jean,Nicolas Poulet,Nicolas Roset,Gordon H. Copp,Philippe Geniez,Didier Pont,Christine Argillier,Jean-Marc Baudoin,Tiphaine Peroux,Alain J. Crivelli,Anthony Olivier,Manon Acqueberge,Matthieu Le Brun,Peter Möller,Eske Willerslev,Tony Dejean +26 more
TL;DR: It is argued that the proposed DNA‐based approach has the potential to become the next‐generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems.
Journal ArticleDOI
Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global eDNA
TL;DR: In this paper, the basic methodology, benefits, and concerns of eDNA metabarcoding, and systematically cover the applications of the method in global ecology thus far, including biodiversity monitoring across all habitats and taxonomic groups, ancient ecosystem reconstruction, plant-pollinator interactions, diet analysis, invasive species detection, pollution responses, and air quality monitoring.
Journal ArticleDOI
Environmental DNA metabarcoding of lake fish communities reflects long-term data from established survey methods.
Bernd Hänfling,Lori Lawson Handley,Daniel S. Read,Christoph Hahn,Jianlong Li,Paul Nichols,Rosetta C. Blackman,Anna Oliver,Ian J. Winfield +8 more
TL;DR: It is demonstrated that eDNA metabarcoding can describe fish communities in large lakes, both qualitatively and quantitatively, and has great potential as a complementary tool to established monitoring methods.
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Related Papers (5)
Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
Alice Valentini,Pierre Taberlet,Pierre Taberlet,Claude Miaud,Raphaël Civade,Jelger Herder,Philip Francis Thomsen,Eva Bellemain,Aurélien Besnard,Eric Coissac,Eric Coissac,Frédéric Boyer,Frédéric Boyer,Coline Gaboriaud,Pauline Jean,Nicolas Poulet,Nicolas Roset,Gordon H. Copp,Gordon H. Copp,Philippe Geniez,Didier Pont,Christine Argillier,Jean-Marc Baudoin,Tiphaine Peroux,Alain J. Crivelli,Anthony Olivier,Manon Acqueberge,Matthieu Le Brun,Peter Rask Møller,Eske Willerslev,Tony Dejean +30 more