Institution
Gulf Coast Regional Blood Center
About: Gulf Coast Regional Blood Center is a based out in . It is known for research contribution in the topics: Population & Allele. The organization has 6297 authors who have published 6917 publications receiving 198369 citations.
Topics: Population, Allele, Human leukocyte antigen, Antigen, Antibody
Papers published on a yearly basis
Papers
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TL;DR: DNA sequencing of the full length VWF coding sequence showed this subject was heterozygous for a mutation in exon 31 (5356C>G) which led to substitution of aspartic acid for the wild-type histidine at amino acid 1786 (H1786D).
73 citations
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TL;DR: The first laboratory‐based blood donor screening program for Babesia microti is developed and implemented to help reduce and prevent transfusion‐transmitted babesiosis (TTB) and report results for the initial year.
73 citations
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TL;DR: Mutation of residues within the putative EF hand loop of human PL scramblase resulted in loss of its PL mobilizing function, suggesting that these residues directly participate in the Ca2+-induced active conformation of the polypeptide.
Abstract: Accelerated transbilayer movement of plasma membrane phospholipids (PL) upon elevation of Ca2+ in the cytosol plays a central role in the initiation of plasma clotting and in phagocytic clearance of injured or apoptotic cells. We recently identified a human erythrocyte membrane protein that induces rapid transbilayer movement of PL at elevated Ca2+. We also presented evidence that this PL scramblase is expressed in a variety of other cells and tissues where transbilayer movement of plasma membrane PL is promoted by intracellular Ca2+ [Zhou, Q., et al. (1997) J. Biol. Chem. 272, 18240-18244]. We have now cloned murine PL scramblase for comparison with the human polypeptide. Both human and murine PL scramblase are acidic proteins (pI = 4.9) with a predicted inside-outside (type 2) transmembrane segment at the carboxyl-terminus. Whereas human PL scramblase (318 AA) terminates in a short exoplasmic tail, murine PL scramblase (307 AA) terminates in the predicted membrane-inserted segment. The aligned polypeptide sequences reveal 65% overall identity, including near identity through 12 residues of an apparent Ca2+ binding motif (D[A/S]DNFGIQFPLD) spanning codons 273-284 (human) and 271-282 (murine), respectively. This conserved sequence in the cytoplasmic domain of PL scramblase shows similarity to Ca2+-binding loop motifs previously identified in known EF hand structures. Recombinant murine and human PL scramblase were each expressed in Escherichia coli and incorporated into proteoliposomes. Measurement of transbilayer movement of NBD-labeled PL confirmed that both proteins catalyzed Ca2+-dependent PL flip-flop similar to that observed for the action of Ca2+ at the cytoplasmic face of plasma membranes. Mutation of residues within the putative EF hand loop of human PL scramblase resulted in loss of its PL mobilizing function, suggesting that these residues directly participate in the Ca2+-induced active conformation of the polypeptide.
73 citations
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TL;DR: Knowledge of the anticoagulant mechanisms and tissue expression patterns of TFPIα and TFPiβ have improved the understanding of the phenotypes observed in different mouse models of T FPI deficiency, the east Texas bleeding disorder, and the development of pharmaceutical agents that block TfpI function to treat hemophilia.
73 citations
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TL;DR: Studies were conducted using samples from early and late‐stage HBV‐infected persons to determine the pool size at which PCR had better sensitivity than a sensitive HBsAg chemoluminescence immunoassay (CLIA‐HBsAg).
72 citations
Authors
Showing all 6297 results
Name | H-index | Papers | Citations |
---|---|---|---|
Martin G. Larson | 171 | 620 | 117708 |
Ernest E. Moore | 132 | 1247 | 73396 |
Jeffery D. Molkentin | 131 | 482 | 61594 |
Mary M. Horowitz | 127 | 557 | 56539 |
Olivier Hermine | 111 | 1026 | 43779 |
Zaverio M. Ruggeri | 104 | 391 | 36417 |
Steven M. Albelda | 103 | 398 | 41200 |
Hans D. Ochs | 102 | 419 | 39881 |
Sanford J. Shattil | 99 | 239 | 30840 |
Michael P. Busch | 96 | 758 | 43075 |
Jinlong Yang | 95 | 765 | 35981 |
Hiroaki Okamoto | 94 | 722 | 39057 |
Irwin D. Bernstein | 89 | 311 | 26624 |
Mark J. Ratain | 88 | 651 | 34779 |
Edgar G. Engleman | 87 | 346 | 28243 |