Institution
Gulf Coast Regional Blood Center
About: Gulf Coast Regional Blood Center is a based out in . It is known for research contribution in the topics: Population & Allele. The organization has 6297 authors who have published 6917 publications receiving 198369 citations.
Topics: Population, Allele, Human leukocyte antigen, Antigen, Antibody
Papers published on a yearly basis
Papers
More filters
••
TL;DR: It is found that the outcome of the diseases underlying DIC and TTP is related to vascular endothelial cells, and that plasma TM, antithrombin, and protein C are useful markers for systemicascular endothelial cell injury.
Abstract: Various hemostatic and vascular endothelial cell markers were measured in patients with disseminated intravascular coagulation (DIC), non-DIC, or thrombotic thrombocytopenic purpura (TTP) and in healthy volunteers to examine the relationships between the hemostatic abnormalities or vascular endothelial cell injuries and the patients' outcomes. Although the plasma levels of soluble fibrin monomer, thrombin-antithrombin complex, plasmin-plasmin inhibitor complex, and D-dimer were significantly increased in the DIC patients, there were no significant differences in these markers between the DIC patients who survived and those who died, suggesting that these markers might not be directly related to the patient outcome. The plasma thrombomodulin (TM) levels in the DIC and TTP patients were significantly higher than those in the healthy volunteers, and the plasma TM levels in the patients who died were significantly higher than those in the patients who survived. These findings showed that the TM level reflected the outcome, and that the outcome of the diseases underlying DIC and TTP might depend on vascular endothelial cell injuries. The plasma protein C and antithrombin activities were markedly reduced in the DIC, non-DIC, and TTP patients who died compared to those who survived. These findings suggest that reduced plasma antithrombin and protein C activities are useful markers of systemic vascular endothelial injuries. Although the plasma tissue factor (TF) levels were significantly increased in the DIC patients, there was no significant difference in the plasma TF levels between the DIC patients who died and those who survived. In conclusion, we found that the outcome of the diseases underlying DIC and TTP is related to vascular endothelial cells, and that plasma TM, antithrombin, and protein C are useful markers for systemic vascular endothelial cell injury.
63 citations
••
TL;DR: An improved and simplified method of genotyping was developed for classifying hepatitis C virus isolates into the five common genotypes by PCR with genotype-specific primers deduced from the core gene, eliminating the cross-reaction of the antisense primer with some HCV isolates of genotype I/1a found by the original method.
Abstract: An improved and simplified method of genotyping was developed for classifying hepatitis C virus (HCV) isolates into the five common genotypes, i.e., I/1a, II/1b, III/2a, IV/2b, and V/3a, by PCR with genotype-specific primers deduced from the core gene. Sense and antisense primers, specific for each of the five common genotypes, were designed by comparison of 319 core gene sequences from HCV isolates of various genotypes from genetic groups 1 to 9. In the first round of PCR, a sequence of 433 bp representing nucleotides 319 to 751 was amplified with universal primers. The second round of PCR was performed with respective sense and antisense primers in two separate reactions, one for the amplification of genotypes I/1a and II/1b and the other for the amplification of genotypes III/2a, IV/2b, and V/3a. The specificity of genotyping was confirmed with a panel of 191 serum samples containing HCV isolates whose core gene sequences were known: 110 serum samples infected with HCV of the five common genotypes and 81 serum samples infected with HCV of other genotypes. The use of sense and antisense primers for genotype II/1b (primers 389 and 492) abolished the cross-reaction of the antisense primer for genotype II/1b (primer 133) with some HCV isolates of genotype I/1a found by our original method. The new method was used for genotyping 130 HCV isolates from Spain, 53 from Brazil, 106 from China, and 30 from Macau. A total of 329 bp of the NS5b region (nucleotides 8279 to 8607) of five isolates from Spain and five isolates from Macau which could not be classified as any of the five common HCV genotypes or genotype 2c were sequenced, and the sequences were compared with those of HCV isolates of known genotypes; two isolates from Spain were deduced to be of genotype 4d and one was deduced to be of genotype 1d, while the remaining two isolates from Spain had novel genotypes in genetic group 2; however, all five isolates from Macau were of genotype 6a.
63 citations
••
TL;DR: The results indicate that the adverse effects of KIR-L-MM-G depend on combination of donor-activating KIR genotype-patient cognate KIR ligand type and no ATG preadministration, thereby suggesting the importance of these factors in UR-HSCT and in leukemia treatment using natural killer (NK) cell alloreactivity.
63 citations
••
TL;DR: The conditions for binding of antibodies to glycosphingolipids separated on a thin-layer plate have been optimized for polyclonal antisera and the method has a broad detection range with low background staining.
63 citations
••
TL;DR: HLA 0 to 2 antigen-mismatched UCBT is a reasonable second alternative donor/stem cell source with a survival outcome similar to that of single-HLA-DRB1- mismatched or other 7 of 8 UBMT.
63 citations
Authors
Showing all 6297 results
Name | H-index | Papers | Citations |
---|---|---|---|
Martin G. Larson | 171 | 620 | 117708 |
Ernest E. Moore | 132 | 1247 | 73396 |
Jeffery D. Molkentin | 131 | 482 | 61594 |
Mary M. Horowitz | 127 | 557 | 56539 |
Olivier Hermine | 111 | 1026 | 43779 |
Zaverio M. Ruggeri | 104 | 391 | 36417 |
Steven M. Albelda | 103 | 398 | 41200 |
Hans D. Ochs | 102 | 419 | 39881 |
Sanford J. Shattil | 99 | 239 | 30840 |
Michael P. Busch | 96 | 758 | 43075 |
Jinlong Yang | 95 | 765 | 35981 |
Hiroaki Okamoto | 94 | 722 | 39057 |
Irwin D. Bernstein | 89 | 311 | 26624 |
Mark J. Ratain | 88 | 651 | 34779 |
Edgar G. Engleman | 87 | 346 | 28243 |