Journal ArticleDOI
A new and rapid colorimetric determination of acetylcholinesterase activity.
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TLDR
A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.About:
This article is published in Biochemical Pharmacology.The article was published on 1961-07-01. It has received 23015 citations till now. The article focuses on the topics: Aryl-acylamidase activity & Thiocholine.read more
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Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes)
TL;DR: The lipid composition of the vesicles shows the high sphingomyelin content characteristic of sheep red cell plasma membranes, but not white cell or platelet membranes, consistent with the conclusion that the vESicles are of reticulocyte origin.
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Synapse loss in frontal cortex biopsies in Alzheimer's disease: Correlation with cognitive severity
TL;DR: The loss of neuronal connectivity, indexed by loss of synapses, predicted the degree of cognitive impairment in the patients who underwent biopsy and indicated a degree of structural change in AD brain not likely to be affected by pharmacotherapy.
Journal ArticleDOI
Diclofenac residues as the cause of vulture population decline in Pakistan
J. Lindsay Oaks,Martin Gilbert,Munir Z. Virani,Richard T. Watson,Carol U. Meteyer,Bruce A. Rideout,H. L. Shivaprasad,Shakeel Ahmed,Muhammad Jamshed Iqbal Chaudhry,Muhammad Arshad,Shahid Mahmood,Ahmad Ali,Aleem Ahmed Khan +12 more
TL;DR: Results are provided that directly correlate residues of the anti-inflammatory drug diclofenac with renal failure and renal failure in the Oriental white-backed vulture (OWBV) and it is proposed that residues of veterinary diclotenac are responsible for the OWBV decline.
Journal ArticleDOI
Molecular and cellular biology of cholinesterases
TL;DR: In this article, the authors propose a method to identify the root cause of a problem.Abbreviations: [2]... ].., [3]
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Depletion of microglia and inhibition of exosome synthesis halt tau propagation
Hirohide Asai,Seiko Ikezu,Satoshi Tsunoda,Maria Medalla,Jennifer I. Luebke,Tarik F. Haydar,Benjamin Wolozin,Oleg Butovsky,Sebastian Kügler,Tsuneya Ikezu +9 more
TL;DR: It is found that depleting microglia dramatically suppressed the propagation of tau and reduced excitability in the dentate gyrus in this mouse model, and this data suggest that microglian involvement in tau propagation and the exosome secretion pathway may be a therapeutic target.
References
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
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The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application.
TL;DR: The present method, which is designed for use with short chain 0-acyl derivatives, is based on the finding that hydroxylamine at an alkaline pH in water rapidly converts acetylcholine stoichiometrically to hydroxamic acid throughout a wide range of ester concentration.
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Die fermentative Spaltung des Acetylcholins
TL;DR: In this paper, a new Methode for Nachweis des Fermentes, das Acetylcholin in Cholin and Essigsaure hydrolysiert, die Cholinesterase, was beschrieben.
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The inhibitory effect of stilbamidine, curare and related compounds and its relationship to the active groups of acetylcholine esterase. Action of stilbamidine upon nerve impulse conduction
TL;DR: The observations add further support for the assumption that the fundamental mechanism underlying axonal conduction and synaptic transmission is the same and that the apparent discrepancies must be attributed to secondary facts as e.g. the inability of certain compounds to penetrate the axonal surface membranes.
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Ultramicro assay of the cholinesterases.
TL;DR: A method is described for the simultaneous determination of cholinesterase activity and protein content in minute quantities of tissue, e.g., 50 μg of nervous tissue or 0.2 μl of human serum, having activities ranging from 0.01 to 0.1 μ M AcCh/hr.