Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
Athanasios Beopoulos,Zuzana Mrozova,Zuzana Mrozova,Marie Therese Le Dall,Ivan Hapala,Seraphim Papanikolaou,Thierry Chardot,Jean-Marc Nicaud +7 more
TLDR
A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolyTica is oriented toward the glycerol pathway, which contains six acyl-coenzyme A oxidases that catalyze the limiting step of peroxisomal β-oxidation.Abstract:
A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolytica is oriented toward the glycerol pathway. To redirect carbon flux toward lipid synthesis, the GUT2 gene, which codes for the glycerol-3-phosphate dehydrogenase isomer, was deleted in Y. lipolytica in this study. This Δgut2 mutant strain demonstrated a threefold increase in lipid accumulation compared to the wild-type strain. However, mobilization of lipid reserves occurred after the exit from the exponential phase due to β-oxidation. Y. lipolytica contains six acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX6 genes, that catalyze the limiting step of peroxisomal β-oxidation. Additional deletion of the POX1 to POX6 genes in the Δgut2 strain led to a fourfold increase in lipid content. The lipid composition of all of the strains tested demonstrated high proportions of FFA. The size and number of the lipid bodies in these strains were shown to be dependent on the lipid composition and accumulation ratio.read more
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Yarrowia lipolytica as a model for bio-oil production
Athanasios Beopoulos,Julien Cescut,Ramdane Haddouche,Jean-Louis Uribelarrea,Carole Molina-Jouve,Jean-Marc Nicaud +5 more
TL;DR: This review describes the coordinated pathways of lipid metabolism, storage and mobilization in this yeast, focusing in particular on the roles and regulation of the various enzymes and organelles involved in these processes.
Journal ArticleDOI
Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.
TL;DR: This work demonstrates the excellent capacity for lipid production by the oleaginous yeast Y. lipolytica and the effects of metabolic engineering of two important steps of the lipid synthesis pathway, which acts to divert flux towards the cholesterol synthesis and creates driving force for TAG synthesis.
Journal ArticleDOI
Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production.
TL;DR: In the first part of this review, the biochemistry of lipid accumulation in the oleaginous microorganisms is depicted, as preferential degradation of the neutral lipid fractions is observed.
Journal ArticleDOI
Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production.
John Blazeck,Andrew Hill,Leqian Liu,Rebecca Knight,Jarrett Miller,Anny Pan,Peter B. Otoupal,Hal S. Alper +7 more
TL;DR: A thorough genotypic and phenotypic optimization of an oleaginous organism to create a strain with significant lipogenesis capability is reported, which advances fundamental understanding of lipogenesis, and non-canonical environmental and intracellular stimuli are demonstrated and uncouple lipogenesis from nitrogen starvation.
Journal ArticleDOI
Oleaginous yeasts for biodiesel: current and future trends in biology and production.
Irnayuli R. Sitepu,Luis A. Garay,Ryan Sestric,David B. Levin,David E. Block,J. Bruce German,Kyria Boundy-Mills +6 more
TL;DR: This review examines aspects of oleaginous yeasts not covered in depth in other recent reviews, and proposes standardized terms for units that describe yeast cell mass and lipid production.
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TL;DR: Examples are presented demonstrating that wild-type and genetically engineered strains of Y. lipolytica can be used for alkane and fatty-acid bioconversion, such as aroma production, for production of SCP and SCO, for citric acid production, in bioremediation, in fine chemistry, for steroid biotransformation, and in food industry.
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