Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte
TLDR
The present review has described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules.Abstract:
The Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules. There have been extensive studies on the mechanism of formation of charged gaseous species by the ESI. Several groups have investigated the origin and implications of the multiple charge states of proteins observed in the ESI-mass spectra of the proteins. The charged analytes produced by ESI can be fragmented by activating them in the gas-phase, and thus tandem mass spectrometry has been developed, which provides very important insights on the structural properties of the molecule. The review will highlight recent developments and emerging directions in this fascinating area of research.read more
Citations
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References
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Journal ArticleDOI
Electrospray ionization for mass spectrometry of large biomolecules
TL;DR: Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.
Journal ArticleDOI
Atmospheric pollution profiles in Mexico City in two different seasons
TL;DR: In this article, a CO2-laser-based photoacoustic spectrometer was used to determine the temporal concentration profile of atmospheric ethene in Mexico City, and the results of this campaign were compared with data obtained in the winter of 2001.
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Methods in Enzymology.
TL;DR: This volume is keyed to high resolution electron microscopy, which is a sophisticated form of structural analysis, but really morphology in a modern guise, the physical and mechanical background of the instrument and its ancillary tools are simply and well presented.
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Disintegration of Water Drops in an Electric Field
TL;DR: In this article, it was shown that a conical interface between two fluids can exist in equilibrium in an electric field, but only when the cone has a semi-vertical angle 49.3$^\circ$.
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