Measurement of Cutaneous Inflammation: Estimation of Neutrophil Content with an Enzyme Marker
TLDR
It is demonstrated that MPO can be used as a marker for skin neutrophil content: it is recoverable from skin in soluble form, and is directly related to neutrophIL number.About:
This article is published in Journal of Investigative Dermatology.The article was published on 1982-03-01 and is currently open access. It has received 3298 citations till now. The article focuses on the topics: Myeloperoxidase.read more
Citations
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Hapten-Induced Model of Chronic Inflammation and Ulceration in the Rat Colon
Gerald P. Morris,Paul L. Beck,Margaret S. Herridge,William T. Depew,Myron R. Szewczuk,John L. Wallace +5 more
TL;DR: A simple and reproducible rat model of chronic colonic inflammation by the intraluminal instillation of a solution containing a "barrier breaker" and a hapten to study the pathophysiology of colonic inflammatory disease in a specifically controlled fashion and to evaluate new treatments potentially applicable to inflammatory bowel disease in humans.
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HIF-1α Is Essential for Myeloid Cell-Mediated Inflammation
Thorsten Cramer,Yuji Yamanishi,Björn E. Clausen,Irmgard Förster,Rafal Pawlinski,Nigel Mackman,Volker H. Haase,Rudolf Jaenisch,Maripat Corr,Victor Nizet,Gary S. Firestein,Hans-Peter Gerber,Napoleone Ferrara,Randall S. Johnson +13 more
TL;DR: It is found that activation of HIF-1alpha is essential for myeloid cell infiltration and activation in vivo through a mechanism independent of VEGF, and its direct regulation of survival and function in the inflammatory microenvironment is demonstrated.
Journal ArticleDOI
Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models
TL;DR: In this paper, an assay was devised to quantitate acute intestinal inflammation based on the assessment of myeloperoxidase activity, an enzyme found in neutrophils and, in much smaller quantities, in monocytes and macrophages.
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Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha
TL;DR: It is reported here that mast cells, which are selectively located at portals of bacterial entry, are important to host defence against bacterial infections.
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Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium.
TL;DR: Measuring the MPO content in preparations of canine neutrophils, which is directly correlated with cell number, allows units of MPO activity to be converted into a tissue neutrophil content that can be used to study the relationship between leukocyte infiltration and myocardial injury.
References
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The glomerular permeability determined by dextran clearance using Sephadex gel filtration.
TL;DR: A method is described for measuring glomerular permeability by dextran clearance using Sephadex gel filtration technique, and clearance is shown to be a function of molecular size, decreasing with increasing molecular weight.
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Single-step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque
TL;DR: Centrifugation of heparinized human blood on discontinuous gradients of Ficoll-Hypaque resulted in the simultaneous separation of mononuclear leukocytes, granulocytes, and erythrocytes with high recovery of each cell type.
Journal ArticleDOI
Pyoderma (echthyma) gangrenosum: clinical and experimental observations in five cases occurring in adults
TL;DR: During the period of observation in each case throughout a series of exacerbations and remissions, it appeared evident that there was a definite parallel relationship between the underlying debilitating disease and the cutaneous manifestations.
Journal ArticleDOI
Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization.
Julius Schultz,Kenneth Kaminker +1 more
TL;DR: The conditions for the preparation of a new cellular particulate in which the peroxidase activity is localized is described; it is heavier than mitochondria, and contains at least 10–30 times more per oxidase than the particulate fractions carrying the cytochrome oxidase activity.
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