Journal ArticleDOI
Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics
TLDR
This review focuses on recent advances in immobilization methodol- ogy for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library sys- tems.Abstract:
Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of re- searchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while re- taining structure and native biological function. In this review, we focus on recent advances in immobilization methodol- ogy for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library sys- tems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.read more
Citations
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Journal ArticleDOI
Immobilization techniques in the fabrication of nanomaterial-based electrochemical biosensors: a review.
TL;DR: Given the unique and favorable qualities of gold nanoparticles, graphene and carbon nanotubes as applied to electrochemical biosensors, a consolidated survey of the different methods of nanomaterial immobilized on transducer surfaces and enzyme immobilization on these species is beneficial and timely.
Journal ArticleDOI
Protein immobilization techniques for microfluidic assays
Do Hyun Kim,Amy E. Herr +1 more
TL;DR: A review of immobilization methods and chemistries, and discusses studies exemplar of key approaches, considers the microfluidics literature from 1997 to present, and describes multifunctional surface coatings that can perform tasks that were, until recently, relegated to multiple functional coatings.
Journal ArticleDOI
Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics.
TL;DR: 3‐Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics
Journal ArticleDOI
Protein engineering for directed immobilization.
TL;DR: In this review, the state of the art of the different protein immobilization strategies that are commonly used today with a special focus on biosensor applications are discussed.
Journal ArticleDOI
Biosensor nanoengineering: Design, operation, and implementation for biomolecular analysis
TL;DR: A revolution in the understanding and synthesis of nanomaterials in the last couple of decades contributed significantly to the development of the biosensors in terms of sensitivity, catalytic activity, biocompatibility, and robustness.
References
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