Tuberculosis Biomarkers: From Diagnosis to Protection
TLDR
Efforts are needed to reduce the gap between the exploratory up-stream identification of candidate biomarkers, and the validation of biomarkers against clear clinical endpoints in different populations, which will need a major commitment from both scientists and funding bodies.Abstract:
New approaches to control tuberculosis (TB) worldwide are needed. In particular, new tools for diagnosis and new biomarkers are required to evaluate both pathogen and host key elements of the response to infection. Non-sputum based diagnostic tests, biomarkers predictive of adequate responsiveness to treatment, and biomarkers of risk of developing active TB disease are major goals. Here, we review the current state of the field. Although reports on new candidate biomarkers are numerous, validation and independent confirmation are rare. Efforts are needed to reduce the gap between the exploratory up-stream identification of candidate biomarkers, and the validation of biomarkers against clear clinical endpoints in different populations. This will need a major commitment from both scientists and funding bodies.read more
Citations
More filters
Journal ArticleDOI
Growth of Mycobacterium tuberculosis in vivo segregates with host macrophage metabolism and ontogeny.
TL;DR: It is hypothesized that different macrophage lineages respond divergently to Mtb infection, with IMs exhibiting nutritional restriction and controlling bacterial growth and AMs representing a more nutritionally permissive environment.
Journal ArticleDOI
Immunometabolism at the interface between macrophages and pathogens.
TL;DR: This Review focuses on the integration of data from existing studies, the identification of challenges in generating and interpreting data from ongoing studies and a discussion of the technologies and tools that are required to best address future questions in the field.
Journal Article
Identification of diagnostic markers for tuberculosis by proteomic fingerprinting of serum. Commentary
Melanie Y. White,Rebekah L. Gundry,Stuart J. Cordwell,Dan Agranoff,Delmiro Fernandez-Reyes,Marios C. Papadopoulos,Sergio A. Rojas,Mark Herbster,Alison Loosemore,Edward Tarelli,Jo Sheldon,Achim Schwenk,Richard Pollok,Charlotte F. J. Rayner,Sanjeev Krishna +14 more
TL;DR: In this paper, a supervised machine-learning approach based on the support vector machine (SVM) was used to obtain a classifier that distinguished between the groups in two independent test sets.
Journal Article
Adaptive Immune Features of Natural Killer Cells
TL;DR: In this paper, the authors used a mouse model of cytomegalovirus infection to show that, like T cells, NK cells bearing the virus-specific Ly49H receptor proliferate 100fold in the spleen and 1,000-fold in liver after infection.
Journal ArticleDOI
First characterization of the CD4 and CD8 T-cell responses to QuantiFERON-TB Plus
Elisa Petruccioli,Teresa Chiacchio,Ilaria Pepponi,Valentina Vanini,Rocco Urso,Gilda Cuzzi,Lucia Barcellini,Daniela Maria Cirillo,Fabrizio Palmieri,Giuseppe Ippolito,Delia Goletti +10 more
TL;DR: In this paper, the authors reported the first characterization of the CD4 and CD8 T-cell response to QFT-plus using flow cytometry and showed that mycobacterium tuberculosis (Mtb) antigen-specific T-cells were associated with active TB (p = 0.01).
References
More filters
Journal ArticleDOI
Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework*
Arthur J. Atkinson,Wayne A. Colburn,Victor G. DeGruttola,David L. DeMets,Gregory J. Downing,Daniel F. Hoth,John A. Oates,Carl C. Peck,Robert T. Schooley,Bert A. Spilker,Janet Woodcock,Scott L. Zeger +11 more
TL;DR: Biomarker measurements provide an avenue for researchers to gain a mechanistic understanding of the differences in clinical response that may be influenced by uncontrolled variables (for example, drug metabolism).
Journal ArticleDOI
Rapid molecular detection of tuberculosis and rifampin resistance.
Catharina Boehme,Pamela Nabeta,Doris Hillemann,Mark P. Nicol,Shubhada Shenai,Fiorella Krapp,Jenny Allen,Rasim Tahirli,Robert Blakemore,Roxana Rustomjee,Ana Milovic,Martin Jones,Sean M. O'Brien,David H. Persing,Sabine Ruesch-Gerdes,Eduardo Gotuzzo,Camilla Rodrigues,David Alland,Mark D. Perkins +18 more
TL;DR: The MTB/RIF test provided sensitive detection of tuberculosis and rifampin resistance directly from untreated sputum in less than 2 hours with minimal hands-on time.
Journal ArticleDOI
An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis
Matthew Berry,Christine M. Graham,Finlay W. McNab,Zhaohui Xu,Susannah Bloch,Tolu Oni,Tolu Oni,Katalin A. Wilkinson,Katalin A. Wilkinson,Romain Banchereau,Jason A. Skinner,Robert J. Wilkinson,Robert J. Wilkinson,Robert J. Wilkinson,Charles T. Quinn,Derek Blankenship,Ranju Dhawan,John J. Cush,Asuncion Mejias,Octavio Ramilo,Onn Min Kon,Virginia Pascual,Jacques Banchereau,Damien Chaussabel,Anne O'Garra +24 more
TL;DR: A hitherto underappreciated role of type I IFN-αβ signalling in the pathogenesis of TB is demonstrated, which has implications for vaccine and therapeutic development and a broad range of transcriptional biomarkers with potential as diagnostic and prognostic tools to combat the TB epidemic are provided.
Journal ArticleDOI
A Prospective Study of the Risk of Tuberculosis among Intravenous Drug Users with Human Immunodeficiency Virus Infection
Peter A. Selwyn,Diana Hartel,Victor A. Lewis,Ellie E. Schoenbaum,Sten H. Vermund,Robert S. Klein,Angela T. Walker,Gerald Friedland +7 more
TL;DR: The data suggest that in HIV-infected persons tuberculosis most often results from the reactivation of latent tuberculous infection; these results lend support to recommendations for the aggressive use of chemoprophylaxis against tuberculosis in patients with HIV infection and a positive PPD test.
Journal ArticleDOI
Adaptive immune features of natural killer cells
TL;DR: A mouse model of cytomegalovirus infection is used to show that, like T cells, NK cells bearing the virus-specific Ly49H receptor proliferate 100-fold in the spleen and 1,000- fold in the liver after infection.
Related Papers (5)
An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis
Matthew Berry,Christine M. Graham,Finlay W. McNab,Zhaohui Xu,Susannah Bloch,Tolu Oni,Tolu Oni,Katalin A. Wilkinson,Katalin A. Wilkinson,Romain Banchereau,Jason A. Skinner,Robert J. Wilkinson,Robert J. Wilkinson,Robert J. Wilkinson,Charles T. Quinn,Derek Blankenship,Ranju Dhawan,John J. Cush,Asuncion Mejias,Octavio Ramilo,Onn Min Kon,Virginia Pascual,Jacques Banchereau,Damien Chaussabel,Anne O'Garra +24 more
A blood RNA signature for tuberculosis disease risk: a prospective cohort study.
Daniel E. Zak,Adam Penn-Nicholson,Thomas J. Scriba,Ethan G. Thompson,Sara Suliman,Lynn M. Amon,Hassan Mahomed,Mzwandile Erasmus,Wendy Whatney,Gregory D. Hussey,Deborah Abrahams,Fazlin Kafaar,Tony Hawkridge,Suzanne Verver,E. Jane Hughes,Martin O. C. Ota,Jayne S. Sutherland,Rawleigh Howe,Hazel M. Dockrell,W. Henry Boom,Bonnie Thiel,Tom H. M. Ottenhoff,Harriet Mayanja-Kizza,Amelia C. Crampin,Katrina Downing,Mark Hatherill,Joe Valvo,Smitha Shankar,Shreemanta K. Parida,Stefan H. E. Kaufmann,Gerhard Walzl,Alan Aderem,Willem A. Hanekom +32 more