K
Kirill A. Datsenko
Researcher at Purdue University
Publications - 43
Citations - 25592
Kirill A. Datsenko is an academic researcher from Purdue University. The author has contributed to research in topics: CRISPR & CRISPR interference. The author has an hindex of 24, co-authored 43 publications receiving 23170 citations. Previous affiliations of Kirill A. Datsenko include Rutgers University.
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Tomoya Baba,Takeshi Ara,Miki Hasegawa,Yuki Takai,Yoshiko Okumura,Miki Baba,Kirill A. Datsenko,Masaru Tomita,Barry L. Wanner,Hirotada Mori,Hirotada Mori +10 more
TL;DR: These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
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Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
Ekaterina Semenova,Matthijs M. Jore,Kirill A. Datsenko,Anna Semenova,Edze R. Westra,Barry L. Wanner,John van der Oost,Stan J. J. Brouns,Konstantin Severinov +8 more
TL;DR: It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.
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Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system
Kirill A. Datsenko,Ksenia Pougach,Anton Tikhonov,Barry L. Wanner,Konstantin Severinov,Konstantin Severinov,Ekaterina Semenova +6 more
TL;DR: It is shown that matching sequences that are no longer able to elicit defense, still guide the CRISPR/Cas acquisition machinery to foreign DNA, thus making the spacer acquisition process adaptive and leading to restoration of CRISpr/Cas-mediated protection.
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Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter.
TL;DR: While the degree of induction varied slightly with the strength of the constitutive promoter, expression was affected most by the arabinose concentration, and two expression systems were developed to allow regulatable control of genes under control of P(BAD.