Independent trapping, manipulation and characterization by an all-optical biophotonics workstation
Hans-Ulrik Ulriksen,Jan Thøgersen,Søren Rud Keiding,Ivan R. Perch-Nielsen,Jeppe Seidelin Dam,Darwin Palima,Henrik Stapelfeldt,Jesper Glückstad +7 more
TLDR
In this paper, a side-view geometry is introduced for accessing trapped particles with optical as well as other types of probe methods independent from the trapping process, which is illustrated by CARS and fluorescence spectroscopy of trapped polystyrene beads.Abstract:
Optical trapping has enabled a multitude of applications focusing, in particular, on non-invasive studies of cellular material. The full potential of optical trapping has, however, not yet been exploited due to restricted access to the trapped samples, caused by high numerical aperture objectives needed to focus the trapping laser beams. Here, we use our recently developed biophotonics workstation to overcome this limitation by introducing probing and spectroscopic characterization of optically trapped particles in a side-view geometry perpendicular to the trapping beams rather than in the traditional top-view geometry parallel to the trapping beams. Our method is illustrated by CARS and fluorescence spectroscopy of trapped polystyrene beads. The side-view geometry opens intriguing possibilities for accessing trapped particles with optical as well as other types of probe methods independent from the trapping process.read more
Citations
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Optical trapping and binding
Richard Bowman,Miles J. Padgett +1 more
TL;DR: The underlying theory of optical traps is outlined, and many of the physical observations that have been made in such systems are explored, including 'optical binding', where trapped objects interact with one another through the trapping light field.
Journal ArticleDOI
Wave-guided optical waveguides.
Darwin Palima,Andrew Rafael Bañas,George Vizsnyiczai,Lóránd Kelemen,Pál Ormos,Jesper Glückstad +5 more
TL;DR: This work fabricate and use two photon polymerization microstructures capable of being optically manipulated into any arbitrary orientation, and therefore has freestanding waveguides, which can be positioned anywhere in the sample at any orientation using optical traps.
Journal ArticleDOI
Light-driven micro-tool equipped with a syringe function
TL;DR: A new type of light robot that is capable of loading and unloading cargo using photothermally induced convection currents within the body of the tool and presents a mode of material transport that may have a significant impact on targeted drug delivery and nanofluidics injection.
Journal ArticleDOI
Gearing up for optical microrobotics: micromanipulation and actuation of synthetic microstructures by optical forces
Darwin Palima,Jesper Glückstad +1 more
TL;DR: In this paper, a review of recent works on the actuation of synthetic microstructures using optical trapping and optical manipulation is presented, which can involve either direct manipulation of nanostructures or structure-mediated approaches where the nanostructure form part of larger structures that are suitable for interfacing with diffraction-limited optical fields.
Journal ArticleDOI
Optical microassembly platform for constructing reconfigurable microenvironments for biomedical studies
Peter John Rodrigo,Lóránd Kelemen,Darwin Palima,Carlo Amadeo Alonzo,Pál Ormos,Jesper Glückstad +5 more
TL;DR: A model platform for constructing versatile microenvironments by fabricating morphologically complex microstructures by two-photon polymerization and then assembling these archetypal building blocks into various configurations using multiple, real-time configurable counterpropagating-beam (CB) traps is demonstrated.
References
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Journal Article
Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
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