Arend H. J. Kolk

TL;DR: A novel method based on strain-dependent hybridization patterns of in vitro-amplified DNA with multiple spacer oligonucleotides was found to differentiate M. bovis from M. tuberculosis, a distinction which is often difficult to make by traditional methods.

TL;DR: The results of the study show that the implementation of an effective system for monitoring sensitivity and specificity is required before the PCR can be used reliably in the diagnosis of tuberculosis.

TL;DR: The DNA sequence of a previously cloned Mycobacterium bovis BCG gene encoding an immunogenic 64-kilodalton protein, MbaA, was reported, supporting the previously observed strong reactivity of human T-cell clones with this, for mycobacteria, common antigen.

TL;DR: During the screening of a Mycobacterium tuberculosis lambda gt-11 gene library with monoclonal antibodies, a recombinant clone was detected which contained a mycobacterial DNA insert that hybridized specifically with DNA of M. tuberculosis complex strains.

TL;DR: An international workshop organized and sponsored by the Immunology of Tuberculosis (IMMTUB) component of the World Health Organization (W.H.O.) Vaccine Development Programme to characterize the specificity and reaction patterns of murine monoclonal antibodies raised against various mycobacteria was held in Geneva, 3 to 5 June 1985.