Marie K. Schwinn

TL;DR: NanoBiT provided a means to measure pharmacology of kinase inhibitors known to induce the interaction between BRAF and CRAF and that the reporter responds reliably and dynamically in cells.

TL;DR: A step‐wise model, involving TET–OGT interactions, promotion of GlcNAcylation, and influence on H3K4me3 via SET1/COMPASS is unveiled, highlighting a novel means by which TETs may induce transcriptional activation.

TL;DR: A combination of greater light intensity with improved spectral resolution results in substantially increased detection sensitivity and dynamic range over current BRET technologies, exhibited in a novel assay developed for analyzing the interactions of bromodomain proteins with chromatin in living cells.

TL;DR: The results provide a mechanistic link between HIF1A and CDK8, two potent oncogenes, in the cellular response to hypoxia, and employ a specific variant of the Mediator complex to stimulate RNAPII elongation.

TL;DR: The ability to efficiently tag endogenous proteins with a small luminescent peptide is demonstrated, allowing sensitive quantitation of the response dynamics in their regulated expression and covalent modifications.