Matthew B. Robers

TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).

TL;DR: A combination of greater light intensity with improved spectral resolution results in substantially increased detection sensitivity and dynamic range over current BRET technologies, exhibited in a novel assay developed for analyzing the interactions of bromodomain proteins with chromatin in living cells.

TL;DR: A BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein that can be readily expressed on the N terminus of GPCRs.

TL;DR: A broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells to demonstrate a novel application of BRET for assessing target engagement within the complex milieu of the intracellular environment.

TL;DR: An energy transfer technique is reported that enabled the first broad-spectrum, equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells and revealed improved target selectivity compared with biochemical measurements.