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Christopher T. Eggers
Researcher at Promega
Publications - 10
Citations - 2334
Christopher T. Eggers is an academic researcher from Promega. The author has contributed to research in topics: Luciferase & Bioluminescence. The author has an hindex of 6, co-authored 10 publications receiving 1625 citations.
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Journal ArticleDOI
Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.
Mary P. Hall,James Unch,Brock Binkowski,Michael P. Valley,Braeden L. Butler,Monika G. Wood,Paul Otto,Kristopher Zimmerman,Gediminas Vidugiris,Thomas Machleidt,Matthew B. Robers,Hélène A Benink,Christopher T. Eggers,Michael R. Slater,Poncho Meisenheimer,Dieter Klaubert,Frank Fan,Lance P. Encell,Keith V. Wood +18 more
TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).
Journal ArticleDOI
NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells
Andrew S. Dixon,Marie K. Schwinn,Mary P. Hall,Kris Zimmerman,Paul Otto,Thomas H. Lubben,Braeden L. Butler,Brock Binkowski,Thomas Machleidt,Thomas A. Kirkland,Monika G. Wood,Christopher T. Eggers,Lance P. Encell,Keith V. Wood +13 more
TL;DR: NanoBiT provided a means to measure pharmacology of kinase inhibitors known to induce the interaction between BRAF and CRAF and that the reporter responds reliably and dynamically in cells.
Journal ArticleDOI
CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide
Marie K. Schwinn,Thomas Machleidt,Kris Zimmerman,Christopher T. Eggers,Andrew S. Dixon,Robin Hurst,Mary P. Hall,Lance P. Encell,Brock Binkowski,Keith V. Wood +9 more
TL;DR: The ability to efficiently tag endogenous proteins with a small luminescent peptide is demonstrated, allowing sensitive quantitation of the response dynamics in their regulated expression and covalent modifications.
Journal ArticleDOI
A luminescent biosensor with increased dynamic range for intracellular cAMP.
Brock Binkowski,Braeden L. Butler,Peter F. Stecha,Christopher T. Eggers,Paul Otto,Kris Zimmerman,Gediminas Vidugiris,Monika G. Wood,Lance P. Encell,Frank Fan,Keith V. Wood +10 more
TL;DR: The utility of the evolved biosensor construct to detect a broad range of receptor activity, together with showing suitability for use in high-throughput screening is demonstrated.
Journal ArticleDOI
The luminescent HiBiT peptide enables selective quantitation of G protein-coupled receptor ligand engagement and internalization in living cells.
Michelle E. Boursier,Sergiy Levin,Kris Zimmerman,Thomas Machleidt,Robin Hurst,Braeden L. Butler,Christopher T. Eggers,Thomas A. Kirkland,Keith V. Wood,Rachel Friedman Ohana +9 more
TL;DR: In this paper, bioluminescent resonance energy transfer is used to quantify dynamic interactions between ligands and their cognate HiBiT-tagged GPCRs through competitive binding with fluorescent tracers.