Keith V. Wood

TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.

TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).

TL;DR: NanoBiT provided a means to measure pharmacology of kinase inhibitors known to induce the interaction between BRAF and CRAF and that the reporter responds reliably and dynamically in cells.

TL;DR: This review addresses recent progress made in the use of bioluminescent assays for HTS, highlighting new detection capabilities brought about by engineering luciferase genes, enzymes, and substrates.

TL;DR: A combination of greater light intensity with improved spectral resolution results in substantially increased detection sensitivity and dynamic range over current BRET technologies, exhibited in a novel assay developed for analyzing the interactions of bromodomain proteins with chromatin in living cells.