Mary P. Hall

TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).

TL;DR: NanoBiT provided a means to measure pharmacology of kinase inhibitors known to induce the interaction between BRAF and CRAF and that the reporter responds reliably and dynamically in cells.

TL;DR: The ability to efficiently tag endogenous proteins with a small luminescent peptide is demonstrated, allowing sensitive quantitation of the response dynamics in their regulated expression and covalent modifications.

TL;DR: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferase, are disclosed in this paper.

TL;DR: NanoLuc substrates with improved solubility and bioavailability, hydrofurimazine and fluorofurimazine, strongly enhance bioluminescence signals in vivo and enable bright dual-color biolUMinescent imaging with AkaLuc and AkaLumine.