Bright Ideas for Chemical Biology
Luke D. Lavis,Ronald T. Raines +1 more
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TLDR
The chemical and photophysical properties of oft-used fluorophores are examined and classic and contemporary examples in which utility has been built upon these scaffolds are highlighted.Abstract:
Small-molecule fluorescent probes embody an essential facet of chemical biology. Although numerous compounds are known, the ensemble of fluorescent probes is based on a modest collection of modular “core” dyes. The elaboration of these dyes with diverse chemical moieties is enabling the precise interrogation of biochemical and biological systems. The importance of fluorescence-based technologies in chemical biology elicits a necessity to understand the major classes of small-molecule fluorophores. Here, we examine the chemical and photophysical properties of oft-used fluorophores and highlight classic and contemporary examples in which utility has been built upon these scaffolds.read more
Citations
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A review of NIR dyes in cancer targeting and imaging.
TL;DR: It is believed these newly developed multifunctional NIR dyes will broaden current concept of tumor targeted imaging and hold promise to make an important contribution to the diagnosis and therapeutics for the treatment of cancer.
Journal ArticleDOI
Perylene Bisimide Dye Assemblies as Archetype Functional Supramolecular Materials
Frank Würthner,Chantu R. Saha-Möller,Benjamin Fimmel,Soichiro Ogi,Pawaret Leowanawat,David Schmidt +5 more
Journal ArticleDOI
A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
BODIPY-based probes for the fluorescence imaging of biomolecules in living cells
TL;DR: Advances in the development of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-based fluorescent probes for biological studies over the past decade are covered.
Journal ArticleDOI
Small-Molecule Two-Photon Probes for Bioimaging Applications
Hwan Myung Kim,Bong Rae Cho +1 more
References
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Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
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