J
John J. Macklin
Researcher at Howard Hughes Medical Institute
Publications - 24
Citations - 5591
John J. Macklin is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Calcium imaging & Two-photon excitation microscopy. The author has an hindex of 16, co-authored 24 publications receiving 4202 citations. Previous affiliations of John J. Macklin include United States Department of the Navy.
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Journal ArticleDOI
Optimization of a GCaMP calcium indicator for neural activity imaging.
Jasper Akerboom,Tsai Wen Chen,Trevor J. Wardill,Lin Tian,Lin Tian,Jonathan S. Marvin,Sevinç Mutlu,Sevinç Mutlu,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Federico Esposti,Bart G. Borghuis,Bart G. Borghuis,Xiaonan Richard Sun,Andrew Gordus,Michael B. Orger,Michael B. Orger,Ruben Portugues,Florian Engert,John J. Macklin,Alessandro Filosa,Aman Aggarwal,Aman Aggarwal,Rex Kerr,Ryousuke Takagi,Sebastian Kracun,Eiji Shigetomi,Baljit S. Khakh,Herwig Baier,Leon Lagnado,Samuel S.-H. Wang,Cornelia I. Bargmann,Bruce E. Kimmel,Vivek Jayaraman,Karel Svoboda,Douglas S. Kim,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +39 more
TL;DR: GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
Journal ArticleDOI
A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
High-performance calcium sensors for imaging activity in neuronal populations and microcompartments
Hod Dana,Hod Dana,Yi Sun,Yi Sun,Boaz Mohar,Brad K. Hulse,Aaron M. Kerlin,Jeremy P. Hasseman,Getahun Tsegaye,Arthur Tsang,Allan M. Wong,Ronak Patel,John J. Macklin,Yang Chen,Arthur Konnerth,Vivek Jayaraman,Loren L. Looger,Eric R. Schreiter,Karel Svoboda,Douglas S. Kim,Douglas S. Kim +20 more
TL;DR: The ‘jGCaMP7’ sensors are four genetically encoded calcium indicators with better sensitivity than state-of-the-art GCaMP6 and specifically improved for applications such as neuropil or wide-field imaging.
Journal ArticleDOI
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Jasper Akerboom,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Lin Tian,Lin Tian,Sebastian Wabnig,Matthias Prigge,Johan Tolö,Andrew Gordus,Michael B. Orger,Michael B. Orger,Kristen E. Severi,John J. Macklin,Ronak Patel,Stefan R. Pulver,Trevor J. Wardill,Trevor J. Wardill,Elisabeth Fischer,Christina Schüler,Tsai Wen Chen,Karen S. Sarkisyan,Jonathan S. Marvin,Cornelia I. Bargmann,Douglas S. Kim,Sebastian Kügler,Leon Lagnado,Peter Hegemann,Alexander Gottschalk,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +31 more
TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.
Journal ArticleDOI
Conjunctive input processing drives feature selectivity in hippocampal CA1 neurons
Katie C. Bittner,Christine Grienberger,Sachin P Vaidya,Aaron D. Milstein,John J. Macklin,Junghyup Suh,Susumu Tonegawa,Jeffrey C. Magee +7 more
TL;DR: In this article, the authors examined the role of this processing in feature selectivity and found that dendritic plateau potentials were produced by an interaction between properly timed input from entorhinal cortex and hippocampal CA3.