R
Ronak Patel
Researcher at Howard Hughes Medical Institute
Publications - 36
Citations - 5486
Ronak Patel is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Calcium imaging & Fluorophore. The author has an hindex of 17, co-authored 32 publications receiving 3825 citations. Previous affiliations of Ronak Patel include Janelia Farm Research Campus & University of Texas Southwestern Medical Center.
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Journal ArticleDOI
A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
Sensitive red protein calcium indicators for imaging neural activity
Hod Dana,Boaz Mohar,Boaz Mohar,Yi Sun,Sujatha Narayan,Andrew Gordus,Jeremy P. Hasseman,Getahun Tsegaye,Graham T Holt,Amy Hu,Deepika Walpita,Ronak Patel,John J. Macklin,Cornelia I. Bargmann,Misha B. Ahrens,Eric R. Schreiter,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +19 more
TL;DR: Improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a) are presented, with sensitivity comparable to GCaMP6, to facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
Journal ArticleDOI
High-performance calcium sensors for imaging activity in neuronal populations and microcompartments
Hod Dana,Hod Dana,Yi Sun,Yi Sun,Boaz Mohar,Brad K. Hulse,Aaron M. Kerlin,Jeremy P. Hasseman,Getahun Tsegaye,Arthur Tsang,Allan M. Wong,Ronak Patel,John J. Macklin,Yang Chen,Arthur Konnerth,Vivek Jayaraman,Loren L. Looger,Eric R. Schreiter,Karel Svoboda,Douglas S. Kim,Douglas S. Kim +20 more
TL;DR: The ‘jGCaMP7’ sensors are four genetically encoded calcium indicators with better sensitivity than state-of-the-art GCaMP6 and specifically improved for applications such as neuropil or wide-field imaging.
Journal ArticleDOI
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Jasper Akerboom,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Lin Tian,Lin Tian,Sebastian Wabnig,Matthias Prigge,Johan Tolö,Andrew Gordus,Michael B. Orger,Michael B. Orger,Kristen E. Severi,John J. Macklin,Ronak Patel,Stefan R. Pulver,Trevor J. Wardill,Trevor J. Wardill,Elisabeth Fischer,Christina Schüler,Tsai Wen Chen,Karen S. Sarkisyan,Jonathan S. Marvin,Cornelia I. Bargmann,Douglas S. Kim,Sebastian Kügler,Leon Lagnado,Peter Hegemann,Alexander Gottschalk,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +31 more
TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.
Journal ArticleDOI
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Jonathan B. Grimm,Anand K. Muthusamy,Yajie Liang,Timothy A. Brown,William C. Lemon,Ronak Patel,Rongwen Lu,John J. Macklin,Philipp J. Keller,Na Ji,Luke D. Lavis +10 more
TL;DR: This work refined and extended the Janelia Fluor strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision.