F
Fritjof Helmchen
Researcher at University of Zurich
Publications - 213
Citations - 27290
Fritjof Helmchen is an academic researcher from University of Zurich. The author has contributed to research in topics: Calcium imaging & Neocortex. The author has an hindex of 62, co-authored 197 publications receiving 24157 citations. Previous affiliations of Fritjof Helmchen include École Polytechnique Fédérale de Lausanne & Max Planck Society.
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Resting Microglial Cells Are Highly Dynamic Surveillants of Brain Parenchyma in Vivo
TL;DR: Using in vivo two-photon imaging in neocortex, it is found that microglial cells are highly active in their presumed resting state, continually surveying their microenvironment with extremely motile processes and protrusions.
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Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
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Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Linda Madisen,Aleena R. Garner,Daisuke Shimaoka,Amy S. Chuong,Nathan C. Klapoetke,Lu Li,Alexander van der Bourg,Yusuke Niino,Ladan Egolf,Claudio Monetti,Hong Gu,Maya Mills,Adrian Cheng,Bosiljka Tasic,Thuc Nghi Nguyen,Susan M. Sunkin,Andrea Benucci,Andras Nagy,Atsushi Miyawaki,Fritjof Helmchen,Ruth M. Empson,Thomas Knöpfel,Edward S. Boyden,R. Clay Reid,Matteo Carandini,Hongkui Zeng +25 more
TL;DR: These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity, and develop driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems.
Journal ArticleDOI
Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo
TL;DR: In this article, the authors found that microglial cells are highly active in their presumed resting state, continually surveying their microenvironment with extremely motile processes and protrusions, switching their behavior from patroling to shielding of the injured site.
Journal ArticleDOI
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
TL;DR: A new, highly selective approach for labeling astrocytes in intact rodent neocortex that allows in vivo imaging using two-photon microscopy and combining this method with calcium indicator loading of cell populations demonstrated distinct calcium dynamics in astroglial and neuronal networks.