Isoprene and acetone concentration profiles during exercise on an ergometer
Julian King,Alexander Kupferthaler,Alexander Kupferthaler,Karl Unterkofler,Karl Unterkofler,Helin Koc,Helin Koc,Helin Koc,Susanne Teschl,Gerald Teschl,Wolfram Miekisch,Wolfram Miekisch,Jochen K. Schubert,Jochen K. Schubert,Hartmann Hinterhuber,Hartmann Hinterhuber,Anton Amann,Anton Amann +17 more
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TLDR
Data appear to favor the hypothesis that short-term effects visible in breath isoprene levels are mainly caused by changes in pulmonary gas exchange patterns rather than fluctuations in endogenous synthesis, and hold great potential in capturing continuous dynamics of non-polar, low-soluble VOCs over a wide measurement range.Abstract:
A real-time recording setup combining exhaled breath volatile organic compound (VOC) measurements by proton transfer reaction-mass spectrometry (PTR-MS) with hemodynamic and respiratory data is presented. Continuous automatic sampling of exhaled breath is implemented on the basis of measured respiratory flow: a flow-controlled shutter mechanism guarantees that only end-tidal exhalation segments are drawn into the mass spectrometer for analysis. Exhaled breath concentration profiles of two prototypic compounds, isoprene and acetone, during several exercise regimes were acquired, reaffirming and complementing earlier experimental findings regarding the dynamic response of these compounds reported by Senthilmohan et al (2000 Redox Rep. 5 151-3) and Karl et al (2001 J. Appl. Physiol. 91 762-70). While isoprene tends to react very sensitively to changes in pulmonary ventilation and perfusion due to its lipophilic behavior and low Henry constant, hydrophilic acetone shows a rather stable behavior. Characteristic (median) values for breath isoprene concentration and molar flow, i.e., the amount of isoprene exhaled per minute are 100 ppb and 29 nmol min(-1), respectively, with some intra-individual day-to-day variation. At the onset of exercise breath isoprene concentration increases drastically, usually by a factor of ∼3-4 within about 1 min. Due to a simultaneous increase in ventilation, the associated rise in molar flow is even more pronounced, leading to a ratio between peak molar flow and molar flow at rest of ∼11. Our setup holds great potential in capturing continuous dynamics of non-polar, low-soluble VOCs over a wide measurement range with simultaneous appraisal of decisive physiological factors affecting exhalation kinetics. In particular, data appear to favor the hypothesis that short-term effects visible in breath isoprene levels are mainly caused by changes in pulmonary gas exchange patterns rather than fluctuations in endogenous synthesis.read more
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A review of the volatiles from the healthy human body
B P J de Lacy Costello,Anton Amann,Anton Amann,Huda Al-Kateb,C. Flynn,Wojciech Filipiak,Wojciech Filipiak,T Khalid,D. Osborne,Norman M. Ratcliffe +9 more
TL;DR: A compendium of all the volatile organic compounds (VOCs) emanating from the human body (the volatolome) is for the first time reported and it is the authors' intention that this database will not only be a useful database of VOCs listed in the literature, but will stimulate further study of V OCs from healthy individuals.
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Volatile organic compounds of lung cancer and possible biochemical pathways.
Marwan Hakim,Yoav Y. Broza,Orna Barash,Nir Peled,Michael Phillips,Anton Amann,Anton Amann,Hossam Haick +7 more
TL;DR: Biochemical Pathways Meggie Hakim,† Yoav Y. Broza,† Orna Barash,† Nir Peled,‡ Michael Phillips, Anton Amann, and Hossam Haick*,† †
Journal ArticleDOI
The human volatilome: volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and saliva
Anton Amann,Anton Amann,Ben de Lacy Costello,Wolfram Miekisch,Jochen K. Schubert,Bogusław Buszewski,Joachim D. Pleil,Norman M. Ratcliffe,Terence H. Risby +8 more
TL;DR: In future studies, combined investigations of a particular compound with regard to human matrices such as breath, urine, saliva and cell culture investigations will lead to novel scientific progress in the field.
Journal ArticleDOI
Noninvasive detection of lung cancer by analysis of exhaled breath
Amel Bajtarevic,Amel Bajtarevic,Clemens Ager,Clemens Ager,Martin Pienz,Martin Pienz,Martin Klieber,Martin Klieber,Konrad Schwarz,Konrad Schwarz,Magdalena Ligor,Magdalena Ligor,Magdalena Ligor,Tomasz Ligor,Tomasz Ligor,Tomasz Ligor,Wojciech Filipiak,Wojciech Filipiak,H. Denz,Michael Fiegl,Wolfgang Hilbe,Wolfgang Weiss,Peter Lukas,Herbert Jamnig,Martin Hackl,Alfred Haidenberger,Bogusław Buszewski,Bogusław Buszewski,Wolfram Miekisch,Wolfram Miekisch,Jochen K. Schubert,Jochen K. Schubert,Anton Amann,Anton Amann +33 more
TL;DR: Exhaled breath analysis is promising to become a future non-invasive lung cancer screening method, but precise identification of compounds observed in exhaled breath of lung cancer patients is necessary and GCMS-SPME is a relatively insensitive method.
Journal ArticleDOI
Assessment, origin, and implementation of breath volatile cancer markers
Hossam Haick,Yoav Y. Broza,Pawel Mochalski,Vera Ruzsanyi,Vera Ruzsanyi,Anton Amann,Anton Amann +6 more
TL;DR: This review presents a list of 115 validated cancer-related VOCs published in the literature during the past decade, and classify them with respect to their "fat-to-blood" and "blood- to-air" partition coefficients, which provide an estimation of the relative concentrations of V OCs in alveolar breath, in blood and in the fat compartments of the human body.
References
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