Frank Fan

TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).

TL;DR: This review addresses recent progress made in the use of bioluminescent assays for HTS, highlighting new detection capabilities brought about by engineering luciferase genes, enzymes, and substrates.

TL;DR: By using a combination of small molecules that regulate multiple signaling pathways, a method is developed to guide human embryonic stem cells to a near-pure population of motor neuron progenitors (MNPs) in 12 days, and an enriched population of functionally mature MNs in an additional 16 days.

TL;DR: Firefly luciferase is genetically engineered to detect specific intermolecular interactions through modulation of its luminescence activity, which will be useful for the sensitive detection of cellular physiology in research and drug discovery.

TL;DR: An energy transfer technique is reported that enabled the first broad-spectrum, equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells and revealed improved target selectivity compared with biochemical measurements.