J
James Unch
Researcher at Promega
Publications - 17
Citations - 1332
James Unch is an academic researcher from Promega. The author has contributed to research in topics: Luciferase & Coelenterazine. The author has an hindex of 7, co-authored 17 publications receiving 1046 citations.
Papers
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Journal ArticleDOI
Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.
Mary P. Hall,James Unch,Brock Binkowski,Michael P. Valley,Braeden L. Butler,Monika G. Wood,Paul Otto,Kristopher Zimmerman,Gediminas Vidugiris,Thomas Machleidt,Matthew B. Robers,Hélène A Benink,Christopher T. Eggers,Michael R. Slater,Poncho Meisenheimer,Dieter Klaubert,Frank Fan,Lance P. Encell,Keith V. Wood +18 more
TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).
Patent
Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
James J. Cali,William J. Daily,Erica Hawkins,Dieter Klaubert,Jianquan Liu,Poncho Meisenheimer,Michael Scurria,John Shultz,James Unch,Keith V. Wood,Wenhui Zhou,Michael P. Valley +11 more
TL;DR: In this article, a method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivatives of a fluorophore is provided.
Journal ArticleDOI
Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase
Galina A. Stepanyuk,James Unch,Natalia P. Malikova,Svetlana V. Markova,John Lee,Eugene S. Vysotski +5 more
TL;DR: The coelenterazine-v bound within coelentazine-binding protein has revealed a greater long-term stability at both 4 and 37 °C, and the possibility for effective application of coelEnterazine- v in various in vitro assays is suggested.
Patent
Methods to quench light from optical reactions
William J. Daily,Erika Hawkins,Dieter Klaubert,Mark McDougall,James Unch,Keith V. Wood,Wenhui Zhou,Ji Zhu +7 more
TL;DR: In this article, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-medication reaction.
Patent
Coelenterazine derivatives and methods of using same
TL;DR: In this article, the coelenterazine derivatives are used as substrate for a non-luminescent enzyme and a pro-substrate for a luminecent enzyme.