G
Gediminas Vidugiris
Researcher at Promega
Publications - 27
Citations - 3793
Gediminas Vidugiris is an academic researcher from Promega. The author has contributed to research in topics: Luciferase & Hydrolase. The author has an hindex of 14, co-authored 27 publications receiving 3088 citations.
Papers
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.
Mary P. Hall,James Unch,Brock Binkowski,Michael P. Valley,Braeden L. Butler,Monika G. Wood,Paul Otto,Kristopher Zimmerman,Gediminas Vidugiris,Thomas Machleidt,Matthew B. Robers,Hélène A Benink,Christopher T. Eggers,Michael R. Slater,Poncho Meisenheimer,Dieter Klaubert,Frank Fan,Lance P. Encell,Keith V. Wood +18 more
TL;DR: A novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics is engineered in mammalian cells by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furimazine).
Patent
RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags
TL;DR: In this article, a method and system for providing point of sale and point-of-delivery and/or distribution of products in a restricted access unit near the customer is presented.
Journal ArticleDOI
A luminescent biosensor with increased dynamic range for intracellular cAMP.
Brock Binkowski,Braeden L. Butler,Peter F. Stecha,Christopher T. Eggers,Paul Otto,Kris Zimmerman,Gediminas Vidugiris,Monika G. Wood,Lance P. Encell,Frank Fan,Keith V. Wood +10 more
TL;DR: The utility of the evolved biosensor construct to detect a broad range of receptor activity, together with showing suitability for use in high-throughput screening is demonstrated.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.