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Daniel J. Simpson
Researcher at Promega
Publications - 22
Citations - 3074
Daniel J. Simpson is an academic researcher from Promega. The author has contributed to research in topics: Nucleic acid & RNA. The author has an hindex of 15, co-authored 22 publications receiving 2674 citations. Previous affiliations of Daniel J. Simpson include Office of Technology Transfer & Woods Hole Oceanographic Institution.
Papers
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Evidence for anoxygenic photosynthesis from the distribution of bacteriochlorophylls in the Black Sea.
TL;DR: It is suggested that anoxygenic photosyn-thesis is a relatively recent phenomenon in the Black Sea initiated by shallowing of the chemocline over the past decade and develop-ment of an anoxic layer devoid of O2 and H2S (ref. 7).
Journal ArticleDOI
Luminogenic cytochrome P450 assays.
James J. Cali,Dongping Ma,Mary Sobol,Daniel J. Simpson,Susan Frackman,Troy Good,William J. Daily,David Liu +7 more
TL;DR: Luminogenic cytochrome P450 (CYP) assays couple CYP enzyme activity to firefly luciferase luminescence in a technology called P450-GloTM (Promega) to offer advantages of speed and safety over HPLC and radiochemical-based methods.
Journal ArticleDOI
HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.
Rachel Friedman Ohana,Lance P. Encell,Kate Zhao,Daniel J. Simpson,Michael R. Slater,Marjeta Urh,Keith V. Wood +6 more
TL;DR: A novel protein tag engineered to enhance expression and solubility of recombinant proteins and to provide efficient protein purification coupled with tag removal, designed to bind rapidly and covalently with a unique synthetic linker to achieve an essentially irreversible attachment.
Patent
pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
Craig E. Smith,Diana L. Holmes,Daniel J. Simpson,Jehoshua Katzenhendler,Rex M. Bitner,Josephine C. Grosch +5 more
TL;DR: In this article, the pH dependent ion exchange matrices are designed to bind to the target nucleic acid at a pH wherein the overall charge of the matrix is positive, and to release as the pH of the surrounding solution is increased.