Georgyi V. Los

TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.

TL;DR: A technology for covalent tethering of organic probes directly to a specially designed reporting protein expressed in live cells, which permits imaging of live cells during long time periods, imaging of fixed cells, and multiplexing with different cell/protein analysis techniques.

TL;DR: It is shown that peroxisomes display an age‐related heterogeneity with respect to their capacity to incorporate newly synthesized proteins, and it is demonstrated that these organelles do not exchange their protein content.

TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.