M
Mark McDougall
Researcher at Promega
Publications - 37
Citations - 2712
Mark McDougall is an academic researcher from Promega. The author has contributed to research in topics: Hydrolase & Substrate (chemistry). The author has an hindex of 17, co-authored 37 publications receiving 2267 citations.
Papers
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Quantitative Live-Cell Kinetic Degradation and Mechanistic Profiling of PROTAC Mode of Action.
Kristin M. Riching,Sarah D. Mahan,Cesear Corona,Mark McDougall,James D Vasta,Matthew B. Robers,Marjeta Urh,Danette L. Daniels +7 more
TL;DR: An innovative, modular live-cell platform utilizing endogenous tagging technologies is presented and applied to monitoring PROTAC-mediated degradation of the bromodomain and extra-terminal family members, showing comprehensive real-time degradation and recovery profiles for each target.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.
SUPPLEMENTARY MATERIAL Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +25 more
Patent
Covalent tethering of functional groups to proteins and substrates therefor
Aldis Darzins,Lance P. Encell,Dieter Klaubert,Georgyi V. Los,Mark McDougall,Keith V. Wood,Monika G. Wood,Chad Zimprich +7 more
TL;DR: In this article, a mutant hydrolase can be fused to a protein of interest, and a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein is provided.