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Robert F. Bulleit
Researcher at Promega
Publications - 27
Citations - 2364
Robert F. Bulleit is an academic researcher from Promega. The author has contributed to research in topics: Substrate (chemistry) & Dehalogenase. The author has an hindex of 11, co-authored 27 publications receiving 1988 citations.
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Homogeneous, Bioluminescent Protease Assays: Caspase-3 as a Model
Martha O'brien,William J. Daily,P. Eric Hesselberth,Richard A. Moravec,Michael Scurria,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +7 more
TL;DR: This strategy for highly sensitive, homogeneous protease assays suitable for high-throughput, automated applications uses peptide-conjugated aminoluciferin as the protease substrate and a firefly luciferase that has been molecularly evolved for increased stability.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.
SUPPLEMENTARY MATERIAL Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +25 more
Journal ArticleDOI
Electrophilic aromatic substituted luciferins as bioluminescent probes for glutathione S-transferase assays.
Wenhui Zhou,John Shultz,Nancy Murphy,Erika Hawkins,Laurent Bernad,Troy Good,Leonard Moothart,Susan Frackman,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +10 more
TL;DR: New highly sensitive latent bioluminescent luciferin substrates were designed and synthesized for monitoring mammalian glutathione S-transferase (GST) and Schistosoma japonicum enzyme activities.