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Chad Zimprich
Researcher at Promega
Publications - 37
Citations - 2998
Chad Zimprich is an academic researcher from Promega. The author has contributed to research in topics: Substrate (chemistry) & Hydrolase. The author has an hindex of 15, co-authored 35 publications receiving 2349 citations.
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Target engagement and drug residence time can be observed in living cells with BRET.
Matthew B. Robers,Melanie Dart,Carolyn C. Woodroofe,Chad Zimprich,Thomas A. Kirkland,Thomas Machleidt,Kevin Kupcho,Sergiy Levin,James Robert Hartnett,Kristopher Zimmerman,Andrew L. Niles,Rachel Friedman Ohana,Danette L. Daniels,Michael R. Slater,Monika G. Wood,Mei Cong,Yi-Qiang Cheng,Keith V. Wood +17 more
TL;DR: A broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells to demonstrate a novel application of BRET for assessing target engagement within the complex milieu of the intracellular environment.
Journal ArticleDOI
Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement
James D Vasta,Cesear Corona,Jennifer Wilkinson,Chad Zimprich,James Robert Hartnett,Morgan R. Ingold,Kristopher Zimmerman,Thomas Machleidt,Thomas A. Kirkland,Kristin G. Huwiler,Rachel Friedman Ohana,Michael R. Slater,Paul Otto,Mei Cong,Carrow I. Wells,Benedict-Tilman Berger,Thomas Hanke,Carina Glas,Carina Glas,Ke Ding,Ke Ding,David H. Drewry,Kilian Huber,Kilian Huber,Timothy M. Willson,Stefan Knapp,Susanne Müller,Poncho Meisenheimer,Frank Fan,Keith V. Wood,Matthew B. Robers +30 more
TL;DR: An energy transfer technique is reported that enabled the first broad-spectrum, equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells and revealed improved target selectivity compared with biochemical measurements.
Journal ArticleDOI
Synergistic effects of GDNF and VEGF on lifespan and disease progression in a familial ALS rat model.
Dan Krakora,Patrick L. Mulcrone,Michael M. Meyer,Christina M. Lewis,Ksenija Bernau,Genevieve Gowing,Genevieve Gowing,Chad Zimprich,Patrick Aebischer,Clive N. Svendsen,Clive N. Svendsen,Masatoshi Suzuki +11 more
TL;DR: A combined ex vivo delivery of GDNF and VEGF in extending survival and protecting neuromuscular junctions and motor neurons showed a strong synergistic effect, which further support ex vivo gene therapy approaches for ALS that target skeletal muscle.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.