The first draft of the pigeonpea genome sequence
Nagendra K. Singh,Deepak K. Gupta,Pawan Kumar Jayaswal,Ajay Kumar Mahato,Sutapa Dutta,Surya Prakash Singh,Shefali Bhutani,Vivek Dogra,Bikram Singh,Giriraj Kumawat,Jitendra Kumar Pal,Awadhesh Pandit,Alok Singh,Hukum Rawal,Akhilesh Kumar,G. Rama Prashat,A. K. Khare,Rekha Yadav,Ranjit S. Raje,Mahendra Narain Singh,Subhojit Datta,B. Fakrudin,Keshav B. Wanjari,Rekha Kansal,Prasanta K. Dash,Pradeep K. Jain,Ramcharan Bhattacharya,Kishor Gaikwad,Trilochan Mohapatra,Ramamurthy Srinivasan,Tilak Raj Sharma +30 more
TLDR
This is the first plant genome sequence completed entirely through a network of Indian institutions led by the Indian Council of Agricultural Research and provides a valuable resource for the pigeonpea variety improvement.Abstract:
Pigeonpea (Cajanus cajan) is an important grain legume of the Indian subcontinent, South-East Asia and East Africa. More than eighty five percent of the world pigeonpea is produced and consumed in India where it is a key crop for food and nutritional security of the people. Here we present the first draft of the genome sequence of a popular pigeonpea variety ‘Asha’. The genome was assembled using long sequence reads of 454 GS-FLX sequencing chemistry with mean read lengths of >550 bp and >10-fold genome coverage, resulting in 510,809,477 bp of high quality sequence. Total 47,004 protein coding genes and 12,511 transposable elements related genes were predicted. We identified 1,213 disease resistance/defense response genes and 152 abiotic stress tolerance genes in the pigeonpea genome that make it a hardy crop. In comparison to soybean, pigeonpea has relatively fewer number of genes for lipid biosynthesis and larger number of genes for cellulose synthesis. The sequence contigs were arranged in to 59,681 scaffolds, which were anchored to eleven chromosomes of pigeonpea with 347 genic-SNP markers of an intra-species reference genetic map. Eleven pigeonpea chromosomes showed low but significant synteny with the twenty chromosomes of soybean. The genome sequence was used to identify large number of hypervariable ‘Arhar’ simple sequence repeat (HASSR) markers, 437 of which were experimentally validated for PCR amplification and high rate of polymorphism among pigeonpea varieties. These markers will be useful for fingerprinting and diversity analysis of pigeonpea germplasm and molecular breeding applications. This is the first plant genome sequence completed entirely through a network of Indian institutions led by the Indian Council of Agricultural Research and provides a valuable resource for the pigeonpea variety improvement.read more
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A reference genome for pea provides insight into legume genome evolution
Jonathan Kreplak,Mohammed-Amin Madoui,Petr Cápal,Petr Novák,Karine Labadie,Grégoire Aubert,Philipp E. Bayer,Krishna K. Gali,Robert A. Syme,Dorrie Main,Anthony Klein,Aurélie Bérard,Iva Vrbová,Cyril Fournier,Leo d’Agata,Caroline Belser,Wahiba Berrabah,Helena Toegelová,Zbyněk Milec,Jan Vrána,HueyTyng Lee,HueyTyng Lee,Ayité Kougbeadjo,Morgane Terezol,Cécile Huneau,Chala J. Turo,Nacer Mohellibi,Pavel Neumann,Matthieu Falque,Karine Gallardo,Rebecca J. McGee,Bunyamin Tar’an,Abdelhafid Bendahmane,Jean-Marc Aury,Jacqueline Batley,Marie-Christine Le Paslier,Noel Ellis,Thomas D. Warkentin,Clarice J. Coyne,Jérôme Salse,David Edwards,Judith Lichtenzveig,Jiří Macas,Jaroslav Doležel,Patrick Wincker,Judith Burstin +45 more
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