Example of Plant Methods format
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Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format
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Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format Example of Plant Methods format
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open access Open Access
recommended Recommended

Plant Methods — Template for authors

Publisher: Springer
Categories Rank Trend in last 3 yrs
Plant Science #28 of 445 up up by 30 ranks
Biotechnology #58 of 282 up up by 7 ranks
Genetics #71 of 325 up up by 53 ranks
journal-quality-icon Journal quality:
High
calendar-icon Last 4 years overview: 530 Published Papers | 3603 Citations
indexed-in-icon Indexed in: Scopus
last-updated-icon Last updated: 05/06/2020
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Related Journals

open access Open Access

Springer

Quality:  
High
CiteRatio: 4.1
SJR: 0.833
SNIP: 0.855
open access Open Access
recommended Recommended

IEEE

Quality:  
High
CiteRatio: 6.4
SJR: 0.745
SNIP: 1.278
open access Open Access

Springer

Quality:  
High
CiteRatio: 6.4
SJR: 1.547
SNIP: 1.162

Journal Performance & Insights

Impact Factor

CiteRatio

Determines the importance of a journal by taking a measure of frequency with which the average article in a journal has been cited in a particular year.

A measure of average citations received per peer-reviewed paper published in the journal.

3.61

14% from 2018

Impact factor for Plant Methods from 2016 - 2019
Year Value
2019 3.61
2018 3.17
2017 4.269
2016 3.51
graph view Graph view
table view Table view

6.8

33% from 2019

CiteRatio for Plant Methods from 2016 - 2020
Year Value
2020 6.8
2019 5.1
2018 5.5
2017 4.8
2016 6.9
graph view Graph view
table view Table view

insights Insights

  • Impact factor of this journal has increased by 14% in last year.
  • This journal’s impact factor is in the top 10 percentile category.

insights Insights

  • CiteRatio of this journal has increased by 33% in last years.
  • This journal’s CiteRatio is in the top 10 percentile category.

SCImago Journal Rank (SJR)

Source Normalized Impact per Paper (SNIP)

Measures weighted citations received by the journal. Citation weighting depends on the categories and prestige of the citing journal.

Measures actual citations received relative to citations expected for the journal's category.

1.35

3% from 2019

SJR for Plant Methods from 2016 - 2020
Year Value
2020 1.35
2019 1.316
2018 1.532
2017 1.885
2016 1.756
graph view Graph view
table view Table view

1.956

43% from 2019

SNIP for Plant Methods from 2016 - 2020
Year Value
2020 1.956
2019 1.365
2018 1.325
2017 1.533
2016 1.329
graph view Graph view
table view Table view

insights Insights

  • SJR of this journal has increased by 3% in last years.
  • This journal’s SJR is in the top 10 percentile category.

insights Insights

  • SNIP of this journal has increased by 43% in last years.
  • This journal’s SNIP is in the top 10 percentile category.

Plant Methods

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Springer

Plant Methods

Approved by publishing and review experts on SciSpace, this template is built as per for Plant Methods formatting guidelines as mentioned in Springer author instructions. The current version was created on and has been used by 522 authors to write and format their manuscripts to this journal.

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Last updated on
04 Jun 2020
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ISSN
1606-8610
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Open Access
Yes
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Sherpa RoMEO Archiving Policy
White faq
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Plagiarism Check
Available via Turnitin
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Endnote Style
Download Available
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Citation Type
Numbered
[25]
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Bibliography Example
Blonder, G.E., Tinkham, M., Klapwijk, T.M.: Transition from metallic to tunneling regimes in superconducting microconstrictions: Excess current, charge imbalance, and supercurrent conversion. Phys. Rev. B 25(7), 4515–4532 (1982)

Top papers written in this journal

open accessOpen access Journal Article DOI: 10.1186/1746-4811-1-13
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants
18 Dec 2005 - Plant Methods

Abstract:

We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformatio... We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes. We give an example of expression of heme-thiolate P450 to demonstrate effectiveness of this system. We have also designed vectors that take advantage of a dual luciferase assay system to analyse promoter sequences or post-transcriptional regulation of gene expression. We have demonstrated their utility by co-expression of putative transcription factors and the promoter sequence of potential target genes and show how orthologous promoter sequences respond to these genes. Finally, we have constructed a vector that has allowed us to investigate design features of hairpin constructs related to their ability to initiate RNA silencing, and have used these tools to study cis-regulatory effect of intron-containing gene constructs. In developing a series of vectors ideally suited to transient expression analysis we have provided a resource that further advances the application of this technology. These minimal vectors are ideally suited to conventional cloning methods and we have used them to demonstrate their flexibility to investigate enzyme activity, transcription regulation and post-transcriptional regulatory processes in transient assays. read more read less

Topics:

Expression vector (57%)57% related to the paper, Functional genomics (56%)56% related to the paper, Regulation of gene expression (55%)55% related to the paper, Vector (molecular biology) (54%)54% related to the paper, RNA silencing (54%)54% related to the paper
View PDF
1,197 Citations
open accessOpen access Journal Article DOI: 10.1186/1746-4811-9-29
The advantages and limitations of trait analysis with GWAS: a review
Arthur Korte1, Ashley Farlow1
22 Jul 2013 - Plant Methods

Abstract:

Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS) present... Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS) present a powerful tool to reconnect this trait back to its underlying genetics. In this review we discuss the biological and statistical considerations that underpin a successful analysis or otherwise. The relevance of biological factors including effect size, sample size, genetic heterogeneity, genomic confounding, linkage disequilibrium and spurious association, and statistical tools to account for these are presented. GWAS can offer a valuable first insight into trait architecture or candidate loci for subsequent validation. read more read less

Topics:

Genome-wide association study (52%)52% related to the paper
View PDF
1,088 Citations
open accessOpen access Journal Article DOI: 10.1186/1746-4811-3-12
Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
Erika Varkonyi-Gasic1, Rongmei Wu1, Marion Wood1, Eric F. Walton1, Roger P. Hellens1
12 Oct 2007 - Plant Methods

Abstract:

MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect miRNAs are very limited in... MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect miRNAs are very limited in terms of throughput, sensitivity and specificity. Here we provide protocols for detection and quantification of miRNAs by RT-PCR. We describe an end-point and real-time looped RT-PCR procedure and demonstrate detection of miRNAs from as little as 20 pg of plant tissue total RNA and from total RNA isolated from as little as 0.1 μl of phloem sap. In addition, we have developed an alternative real-time PCR assay that can further improve specificity when detecting low abundant miRNAs. Using this assay, we have demonstrated that miRNAs are differentially expressed in the phloem sap and the surrounding vascular tissue. This method enables fast, sensitive and specific miRNA expression profiling and is suitable for facilitation of high-throughput detection and quantification of miRNA expression. read more read less
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982 Citations
open accessOpen access Journal Article DOI: 10.1186/1746-4811-5-16
Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
Fu Hui Wu1, Shu Chen Shen1, Lan-Ying Lee2, Shu Hong Lee1, Ming Tsar Chan1, Choun-Sea Lin1
24 Nov 2009 - Plant Methods

Abstract:

Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This ... Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts, a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments. In this report, we present a new procedure that we call the Tape-Arabidopsis Sandwich. This is a simple and fast mesophyll protoplast isolation method. Two kinds of tape (Time tape adhered to the upper epidermis and 3 M Magic tape to the lower epidermis) are used to make a "Tape-Arabidopsis Sandwich". The Time tape supports the top side of the leaf during manipulation, while tearing off the 3 M Magic tape allows easy removal of the lower epidermal layer and exposes mesophyll cells to cell wall digesting enzymes when the leaf is later incubated in an enzyme solution. The protoplasts released into solution are collected and washed for further use. For TEAMP, plasmids carrying a gene expression cassette for a fluorescent protein can be successfully delivered into protoplasts isolated from mature leaves grown under optimal conditions. Alternatively, these protoplasts may be used for bimolecular fluorescence complementation (BiFC) to investigate protein-protein interactions in vivo, or for Western blot analysis. A significant advantage of this protocol over the current method is that it allows the generation of protoplasts in less than 1 hr, and allows TEAMP transfection to be carried out within 2 hr. The protoplasts generated by this new Tape-Arabidopsis Sandwich method are suitable for the same range of research applications as those that use the current method, but require less operator skill, equipment and time. read more read less

Topics:

Protoplast (53%)53% related to the paper
View PDF
725 Citations
open accessOpen access Journal Article DOI: 10.1186/1746-4811-7-30
A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes
Yang Zhang1, Jianbin Su1, Duan Shan1, Ying Ao1, Jin-Ran Dai1, Jun Liu1, Peng Wang1, Yuge Li1, Bing Liu1, Dongru Feng1, Jinfa Wang1, Hong-Bin Wang1
30 Sep 2011 - Plant Methods

Abstract:

Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice... Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice, protoplasts are commonly prepared from suspension cultured cells or etiolated seedlings, but only a few studies have explored the use of protoplasts from rice green tissue. Here, we report a simplified method for isolating protoplasts from normally cultivated young rice green tissue without the need for unnecessary chemicals and a vacuum device. Transfections of the generated protoplasts with plasmids of a wide range of sizes (4.5-13 kb) and co-transfections with multiple plasmids achieved impressively high efficiencies and allowed evaluations by 1) protein immunoblotting analysis, 2) subcellular localization assays, and 3) protein-protein interaction analysis by bimolecular fluorescence complementation (BiFC) and firefly luciferase complementation (FLC). Importantly, the rice green tissue protoplasts were photosynthetically active and sensitive to the retrograde plastid signaling inducer norflurazon (NF). Transient expression of the GFP-tagged light-related transcription factor OsGLK1 markedly upregulated transcript levels of the endogeneous photosynthetic genes OsLhcb1, OsLhcp, GADPH and RbcS, which were reduced to some extent by NF treatment in the rice green tissue protoplasts. We show here a simplified and highly efficient transient gene expression system using photosynthetically active rice green tissue protoplasts and its broad applications in protein immunoblot, localization and protein-protein interaction assays. These rice green tissue protoplasts will be particularly useful in studies of light/chloroplast-related processes. read more read less

Topics:

Bimolecular fluorescence complementation (51%)51% related to the paper
View PDF
642 Citations
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Frequently asked questions

1. Can I write Plant Methods in LaTeX?

Absolutely not! Our tool has been designed to help you focus on writing. You can write your entire paper as per the Plant Methods guidelines and auto format it.

2. Do you follow the Plant Methods guidelines?

Yes, the template is compliant with the Plant Methods guidelines. Our experts at SciSpace ensure that. If there are any changes to the journal's guidelines, we'll change our algorithm accordingly.

3. Can I cite my article in multiple styles in Plant Methods?

Of course! We support all the top citation styles, such as APA style, MLA style, Vancouver style, Harvard style, and Chicago style. For example, when you write your paper and hit autoformat, our system will automatically update your article as per the Plant Methods citation style.

4. Can I use the Plant Methods templates for free?

Sign up for our free trial, and you'll be able to use all our features for seven days. You'll see how helpful they are and how inexpensive they are compared to other options, Especially for Plant Methods.

5. Can I use a manuscript in Plant Methods that I have written in MS Word?

Yes. You can choose the right template, copy-paste the contents from the word document, and click on auto-format. Once you're done, you'll have a publish-ready paper Plant Methods that you can download at the end.

6. How long does it usually take you to format my papers in Plant Methods?

It only takes a matter of seconds to edit your manuscript. Besides that, our intuitive editor saves you from writing and formatting it in Plant Methods.

7. Where can I find the template for the Plant Methods?

It is possible to find the Word template for any journal on Google. However, why use a template when you can write your entire manuscript on SciSpace , auto format it as per Plant Methods's guidelines and download the same in Word, PDF and LaTeX formats? Give us a try!.

8. Can I reformat my paper to fit the Plant Methods's guidelines?

Of course! You can do this using our intuitive editor. It's very easy. If you need help, our support team is always ready to assist you.

9. Plant Methods an online tool or is there a desktop version?

SciSpace's Plant Methods is currently available as an online tool. We're developing a desktop version, too. You can request (or upvote) any features that you think would be helpful for you and other researchers in the "feature request" section of your account once you've signed up with us.

10. I cannot find my template in your gallery. Can you create it for me like Plant Methods?

Sure. You can request any template and we'll have it setup within a few days. You can find the request box in Journal Gallery on the right side bar under the heading, "Couldn't find the format you were looking for like Plant Methods?”

11. What is the output that I would get after using Plant Methods?

After writing your paper autoformatting in Plant Methods, you can download it in multiple formats, viz., PDF, Docx, and LaTeX.

12. Is Plant Methods's impact factor high enough that I should try publishing my article there?

To be honest, the answer is no. The impact factor is one of the many elements that determine the quality of a journal. Few of these factors include review board, rejection rates, frequency of inclusion in indexes, and Eigenfactor. You need to assess all these factors before you make your final call.

13. What is Sherpa RoMEO Archiving Policy for Plant Methods?

SHERPA/RoMEO Database

We extracted this data from Sherpa Romeo to help researchers understand the access level of this journal in accordance with the Sherpa Romeo Archiving Policy for Plant Methods. The table below indicates the level of access a journal has as per Sherpa Romeo's archiving policy.

RoMEO Colour Archiving policy
Green Can archive pre-print and post-print or publisher's version/PDF
Blue Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
Yellow Can archive pre-print (ie pre-refereeing)
White Archiving not formally supported
FYI:
  1. Pre-prints as being the version of the paper before peer review and
  2. Post-prints as being the version of the paper after peer-review, with revisions having been made.

14. What are the most common citation types In Plant Methods?

The 5 most common citation types in order of usage for Plant Methods are:.

S. No. Citation Style Type
1. Author Year
2. Numbered
3. Numbered (Superscripted)
4. Author Year (Cited Pages)
5. Footnote

15. How do I submit my article to the Plant Methods?

It is possible to find the Word template for any journal on Google. However, why use a template when you can write your entire manuscript on SciSpace , auto format it as per Plant Methods's guidelines and download the same in Word, PDF and LaTeX formats? Give us a try!.

16. Can I download Plant Methods in Endnote format?

Yes, SciSpace provides this functionality. After signing up, you would need to import your existing references from Word or Bib file to SciSpace. Then SciSpace would allow you to download your references in Plant Methods Endnote style according to Elsevier guidelines.

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