Elisabeth Fischer

TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.

TL;DR: Highly light-sensitive optogenetic tools are introduced, bypassing previous shortcomings, and thus constituting new tools that feature high effectiveness and fast kinetics, allowing better repetitive stimulation or investigating prolonged neuronal activity states in Caenorhabditis elegans and, possibly, other systems.

TL;DR: A model may allow screening of drug candidates affecting specific VGCCs mutations, and permit to better understand the effects of distinct mutations on a macroscopic level, to enable drug development for distinct arrhythmias.

TL;DR: The ‘worm arrhythmia’ of Caenorhabditis elegans is reversed by the benzothiazepine S107, establishing the nematode pharynx for studying specific CPVT mutations and for drug screening.