L
Leon Lagnado
Researcher at University of Sussex
Publications - 111
Citations - 8896
Leon Lagnado is an academic researcher from University of Sussex. The author has contributed to research in topics: Exocytosis & Synaptic vesicle. The author has an hindex of 44, co-authored 104 publications receiving 8148 citations. Previous affiliations of Leon Lagnado include Laboratory of Molecular Biology & Medical Research Council.
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Journal ArticleDOI
Optimization of a GCaMP calcium indicator for neural activity imaging.
Jasper Akerboom,Tsai Wen Chen,Trevor J. Wardill,Lin Tian,Lin Tian,Jonathan S. Marvin,Sevinç Mutlu,Sevinç Mutlu,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Federico Esposti,Bart G. Borghuis,Bart G. Borghuis,Xiaonan Richard Sun,Andrew Gordus,Michael B. Orger,Michael B. Orger,Ruben Portugues,Florian Engert,John J. Macklin,Alessandro Filosa,Aman Aggarwal,Aman Aggarwal,Rex Kerr,Ryousuke Takagi,Sebastian Kracun,Eiji Shigetomi,Baljit S. Khakh,Herwig Baier,Leon Lagnado,Samuel S.-H. Wang,Cornelia I. Bargmann,Bruce E. Kimmel,Vivek Jayaraman,Karel Svoboda,Douglas S. Kim,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +39 more
TL;DR: GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
Journal ArticleDOI
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Jasper Akerboom,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Lin Tian,Lin Tian,Sebastian Wabnig,Matthias Prigge,Johan Tolö,Andrew Gordus,Michael B. Orger,Michael B. Orger,Kristen E. Severi,John J. Macklin,Ronak Patel,Stefan R. Pulver,Trevor J. Wardill,Trevor J. Wardill,Elisabeth Fischer,Christina Schüler,Tsai Wen Chen,Karen S. Sarkisyan,Jonathan S. Marvin,Cornelia I. Bargmann,Douglas S. Kim,Sebastian Kügler,Leon Lagnado,Peter Hegemann,Alexander Gottschalk,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +31 more
TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.
Journal ArticleDOI
Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses.
TL;DR: This article showed that clathrin-mediated endocytosis is the major mechanism of vesicle retrieval after physiological stimuli, and that this mechanism is blocked by over-expression of the C-terminal fragment of AP180 or by knockdown of Clathrin using RNAi.
Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses
TL;DR: Results indicate that clathrin-mediated endocytosis is the major, if not exclusive, mechanism of vesicle retrieval after physiological stimuli.
Journal ArticleDOI
Concerted Signaling by Retinal Ganglion Cells
TL;DR: To analyze the rules that govern communication between eye and brain, visual responses were recorded from an intact salamander retina and concerted spikes encoded information not carried by individual cells; they may represent symbols in a multineuronal code for vision.