T
Timothy T. Harkins
Researcher at Life Technologies
Publications - 82
Citations - 15373
Timothy T. Harkins is an academic researcher from Life Technologies. The author has contributed to research in topics: Genome & Deep sequencing. The author has an hindex of 43, co-authored 79 publications receiving 13693 citations. Previous affiliations of Timothy T. Harkins include Thermo Fisher Scientific & Roche Applied Science.
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Journal ArticleDOI
Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies
Chetan Bettegowda,Chetan Bettegowda,Mark Sausen,Rebecca J. Leary,Isaac Kinde,Yuxuan Wang,Nishant Agrawal,Nishant Agrawal,Bjarne Bartlett,Bjarne Bartlett,Hao Wang,Brandon Luber,Rhoda M. Alani,Emmanuel S. Antonarakis,Nilofer S. Azad,Alberto Bardelli,Henry Brem,John L. Cameron,Clarence Lee,Leslie A. Fecher,Leslie A. Fecher,Gary L. Gallia,Peter Gibbs,Dung T. Le,Dung T. Le,Robert L. Giuntoli,Michael Goggins,Michael D. Hogarty,Matthias Holdhoff,Seung-Mo Hong,Seung-Mo Hong,Yuchen Jiao,Hartmut Juhl,Jenny J. Kim,Giulia Siravegna,Daniel A. Laheru,Calogero Lauricella,Michael Lim,Evan J. Lipson,Suely Kazue Nagahashi Marie,George J. Netto,Kelly S. Oliner,Alessandro Olivi,Louise Olsson,Gregory J. Riggins,Andrea Sartore-Bianchi,Kerstin Schmidt,le-Ming Shih,Sueli Mieko Oba-Shinjo,Salvatore Siena,Dan Theodorescu,Jeanne Tie,Timothy T. Harkins,Silvio Veronese,Tian Li Wang,Jon D. Weingart,Christopher L. Wolfgang,Laura D. Wood,Dongmei Xing,Ralph H. Hruban,Jian Wu,Peter J. Allen,C. Max Schmidt,Michael A. Choti,Victor E. Velculescu,Kenneth W. Kinzler,Bert Vogelstein,Nickolas Papadopoulos,Luis A. Diaz,Luis A. Diaz +69 more
TL;DR: The ability of circulating tumor DNA (ctDNA) to detect tumors in 640 patients with various cancer types was evaluated and suggested that ctDNA is a broadly applicable, sensitive, and specific biomarker that can be used for a variety of clinical and research purposes.
Journal ArticleDOI
The clonal and mutational evolution spectrum of primary triple-negative breast cancers
Sohrab P. Shah,Andrew Roth,Rodrigo Goya,Arusha Oloumi,Gavin Ha,Yongjun Zhao,Gulisa Turashvili,Jiarui Ding,Kane Tse,Gholamreza Haffari,Ali Bashashati,Leah M Prentice,Jaswinder Khattra,Angela Burleigh,Damian Yap,Virginie Bernard,Andrew McPherson,Karey Shumansky,Anamaria Crisan,Ryan Giuliany,Alireza Heravi-Moussavi,Jamie Rosner,Daniel Lai,Inanc Birol,Richard Varhol,Angela Tam,Noreen Dhalla,Thomas Zeng,Kevin C. Ma,Simon K. Chan,Malachi Griffith,Annie Moradian,S.-W. Grace Cheng,Gregg B. Morin,Peter H. Watson,Karen A. Gelmon,Stephen Chia,Suet-Feung Chin,Christina Curtis,Christina Curtis,Oscar M. Rueda,Paul D.P. Pharoah,Sambasivarao Damaraju,John R. Mackey,Kelly Hoon,Timothy T. Harkins,Vasisht Tadigotla,Mahvash Sigaroudinia,Philippe Gascard,Thea D. Tlsty,Joseph F. Costello,Irmtraud M. Meyer,Connie J. Eaves,Wyeth W. Wasserman,Steven J.M. Jones,Steven J.M. Jones,David G. Huntsman,David G. Huntsman,Martin Hirst,Carlos Caldas,Marco A. Marra,Samuel Aparicio +61 more
TL;DR: It is shown that understanding the biology and therapeutic responses of patients with TNBC will require the determination of individual tumour clonal genotypes, and for the first time in an epithelial tumour subtype, the relative abundance of clonal frequencies among cases representative of the population is determined.
Journal ArticleDOI
Paired-end mapping reveals extensive structural variation in the human genome.
Jan O. Korbel,Alexander E. Urban,Jason P. Affourtit,Brian C. Godwin,Fabian Grubert,Jan Fredrik Simons,Philip M. Kim,Dean Palejev,Nicholas Carriero,Lei Du,Bruce E. Taillon,Zhoutao Chen,Andrea Tanzer,A. C. Eugenia Saunders,Jianxiang Chi,Fengtang Yang,Nigel P. Carter,Matthew E. Hurles,Sherman M. Weissman,Timothy T. Harkins,Mark Gerstein,Michael Egholm,Michael Snyder +22 more
TL;DR: High-throughput and massive paired-end mapping (PEM) was used to map SVs in an African and in a putatively European individual and identified shared and divergent SVs relative to the reference genome, documenting that the number of SVs among humans is much larger than initially hypothesized; many of the SVs potentially affect gene function.
Journal ArticleDOI
Spatial and temporal diversity in genomic instability processes defines lung cancer evolution
Elza C de Bruin,Nicholas McGranahan,Nicholas McGranahan,Richard Mitter,Max Salm,David C. Wedge,Lucy R. Yates,Lucy R. Yates,Mariam Jamal-Hanjani,Seema Shafi,Nirupa Murugaesu,Andrew Rowan,Eva Grönroos,Madiha A. Muhammad,Stuart Horswell,Marco Gerlinger,Ignacio Varela,David T. Jones,John Marshall,Thierry Voet,Thierry Voet,Peter Van Loo,Peter Van Loo,Doris Rassl,Robert C. Rintoul,Sam M. Janes,Siow Ming Lee,Martin Forster,Tanya Ahmad,David Lawrence,Mary Falzon,Arrigo Capitanio,Timothy T. Harkins,Clarence C. Lee,Warren Tom,Enock Teefe,Shann-Ching Chen,Sharmin Begum,Adam Rabinowitz,Benjamin Phillimore,Bradley Spencer-Dene,Gordon Stamp,Zoltan Szallasi,Zoltan Szallasi,Nik Matthews,Aengus Stewart,Peter J. Campbell,Charles Swanton,Charles Swanton +48 more
TL;DR: 25 spatially distinct regions from seven operable NSCLCs were sequenced and found evidence of branched evolution, with driver mutations arising before and after subclonal diversification, and pronounced intratumor heterogeneity in copy number alterations, translocations, and mutations associated with APOBEC cytidine deaminase activity.
Journal ArticleDOI
Genome Sequencing Highlights the Dynamic Early History of Dogs
Adam H. Freedman,Ilan Gronau,Rena M. Schweizer,Diego Ortega-Del Vecchyo,Eunjung Han,Pedro Silva,Marco Galaverni,Zhenxin Fan,Peter Marx,Belen Lorente-Galdos,Holly C. Beale,Oscar Ramirez,Fereydoun Hormozdiari,Can Alkan,Carles Vilà,Kevin Squire,Eli Geffen,Josip Kusak,Adam R. Boyko,Heidi G. Parker,Clarence Lee,Vasisht Tadigotla,Adam Siepel,Carlos Bustamante,Timothy T. Harkins,Stanley F. Nelson,Elaine A. Ostrander,Tomas Marques-Bonet,Tomas Marques-Bonet,Robert K. Wayne,John Novembre +30 more
TL;DR: It is found that none of the extant wolf lineages from putative domestication centers is more closely related to dogs, and, instead, the sampled wolves form a sister monophyletic clade, suggesting that a re-evaluation of past hypotheses regarding dog origins is necessary.