A GAL4-Driver Line Resource for Drosophila Neurobiology
Arnim Jenett,Gerald M. Rubin,Teri-T B. Ngo,David Shepherd,Christine Murphy,Heather Dionne,Barret D. Pfeiffer,Amanda Cavallaro,Donald Hall,Jennifer Jeter,Nirmala Iyer,Dona Fetter,Joanna H. Hausenfluck,Hanchuan Peng,Eric T. Trautman,Robert Svirskas,Eugene W. Myers,Zbigniew R. Iwinski,Yoshinori Aso,Gina M. DePasquale,Adrianne Enos,Phuson Hulamm,S. Lam,Hsing-Hsi Li,Todd R. Laverty,Fuhui Long,Lei Qu,Sean D. Murphy,Konrad Rokicki,Todd Safford,Kshiti Shaw,Julie H. Simpson,Allison Sowell,Susana Tae,Yang Yu,Christopher T. Zugates +35 more
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TLDR
The utility of 7,000 transgenic lines of Drosophila melanogaster for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy is illustrated.About:
This article is published in Cell Reports.The article was published on 2012-10-25 and is currently open access. It has received 1274 citations till now. The article focuses on the topics: Drosophila Protein.read more
Citations
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A mesoscale connectome of the mouse brain
Seung Wook Oh,Julie A. Harris,Lydia Ng,Brent Winslow,Nicholas Cain,Stefan Mihalas,Quanxin Wang,Chris Lau,Leonard Kuan,Alex M. Henry,Marty Mortrud,Benjamin Ouellette,Thuc Nghi Nguyen,Staci A. Sorensen,Clifford R. Slaughterbeck,Wayne Wakeman,Yang Li,David Feng,Anh Ho,Eric Nicholas,Karla E. Hirokawa,Phillip Bohn,Kevin M. Joines,Hanchuan Peng,Michael Hawrylycz,John W. Phillips,John G. Hohmann,Paul Wohnoutka,Charles R. Gerfen,Christof Koch,Amy Bernard,Chinh Dang,Allan R. Jones,Hongkui Zeng +33 more
TL;DR: A brain-wide, cellular-level, mesoscale connectome for the mouse, using enhanced green fluorescent protein-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain.
Journal ArticleDOI
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Linda Madisen,Aleena R. Garner,Daisuke Shimaoka,Amy S. Chuong,Nathan C. Klapoetke,Lu Li,Alexander van der Bourg,Yusuke Niino,Ladan Egolf,Claudio Monetti,Hong Gu,Maya Mills,Adrian Cheng,Bosiljka Tasic,Thuc Nghi Nguyen,Susan M. Sunkin,Andrea Benucci,Andras Nagy,Atsushi Miyawaki,Fritjof Helmchen,Ruth M. Empson,Thomas Knöpfel,Edward S. Boyden,R. Clay Reid,Matteo Carandini,Hongkui Zeng +25 more
TL;DR: These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity, and develop driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems.
Journal ArticleDOI
The neuronal architecture of the mushroom body provides a logic for associative learning
Yoshinori Aso,Daisuke Hattori,Yang Yu,Rebecca M. Johnston,Nirmala Iyer,Teri T.B. Ngo,Heather Dionne,Larry F. Abbott,Richard Axel,Hiromu Tanimoto,Gerald M. Rubin +10 more
TL;DR: The elucidation of the complement of neurons of the MB provides a comprehensive anatomical substrate from which one can infer a functional logic of associative olfactory learning and memory.
Journal ArticleDOI
High-performance calcium sensors for imaging activity in neuronal populations and microcompartments
Hod Dana,Hod Dana,Yi Sun,Yi Sun,Boaz Mohar,Brad K. Hulse,Aaron M. Kerlin,Jeremy P. Hasseman,Getahun Tsegaye,Arthur Tsang,Allan M. Wong,Ronak Patel,John J. Macklin,Yang Chen,Arthur Konnerth,Vivek Jayaraman,Loren L. Looger,Eric R. Schreiter,Karel Svoboda,Douglas S. Kim,Douglas S. Kim +20 more
TL;DR: The ‘jGCaMP7’ sensors are four genetically encoded calcium indicators with better sensitivity than state-of-the-art GCaMP6 and specifically improved for applications such as neuropil or wide-field imaging.
Journal ArticleDOI
A visual motion detection circuit suggested by Drosophila connectomics
Shin-ya Takemura,Arjun Bharioke,Zhiyuan Lu,Zhiyuan Lu,Aljoscha Nern,Shiv Naga Prasad Vitaladevuni,Patricia K. Rivlin,William T. Katz,Donald J. Olbris,Stephen M. Plaza,Philip Brown Winston,Ting Zhao,Jane Anne Horne,Richard D. Fetter,Satoko Takemura,Katerina Blazek,Lei-Ann Chang,Omotara Ogundeyi,Mathew A. Saunders,Victor Shapiro,Christopher Sigmund,Gerald M. Rubin,Louis K. Scheffer,Ian A. Meinertzhagen,Ian A. Meinertzhagen,Dmitri B. Chklovskii +25 more
TL;DR: A semi-automated pipeline using electron microscopy is developed to reconstruct a connectome, containing 379 neurons and 8,637 chemical synaptic contacts, within the Drosophila optic medulla, and it is demonstrated that connectomes can provide key insights into neuronal computations.
References
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Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TL;DR: The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.
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Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.
TL;DR: The phiC31 integrase injected into embryos as mRNA functioned to promote integration of an attB-containing plasmid into the attP site, resulting in up to 55% of fertile adults producing transgenic offspring.
Journal ArticleDOI
Refinement of Tools for Targeted Gene Expression in Drosophila
Barret D. Pfeiffer,Teri-T B. Ngo,Karen L. Hibbard,Christine Murphy,Arnim Jenett,James W Truman,Gerald M. Rubin +6 more
TL;DR: The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication in Drosophila melanogaster.
Journal ArticleDOI
Tools for neuroanatomy and neurogenetics in Drosophila
Barret D. Pfeiffer,Arnim Jenett,Ann S. Hammonds,Teri T.B. Ngo,Sima Misra,Christine Murphy,Audra Scully,Joseph W. Carlson,Kenneth H. Wan,Todd R. Laverty,Christopher J. Mungall,Rob Svirskas,James T. Kadonaga,Chris Q. Doe,Michael B. Eisen,Susan E. Celniker,Gerald M. Rubin +16 more
TL;DR: The results suggest that the D. melanogaster genome contains >50,000 enhancers and that multiple enhancers drive distinct subsets of expression of a gene in each tissue and developmental stage.
Journal ArticleDOI
Bruchpilot, a Protein with Homology to ELKS/CAST, Is Required for Structural Integrity and Function of Synaptic Active Zones in Drosophila
Dhananjay A. Wagh,Tobias M. Rasse,Esther Asan,Alois Hofbauer,Isabell Schwenkert,Heike Dürrbeck,Sigrid Buchner,Marie-Christine Dabauvalle,Manuela Schmidt,Gang Qin,Carolin Wichmann,Robert J. Kittel,Stephan J. Sigrist,Erich Buchner +13 more
TL;DR: It is proposed that Bruchpilot protein (BRP) is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila.
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