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Giovanni Miotto

Researcher at University of Padua

Publications -  65
Citations -  5540

Giovanni Miotto is an academic researcher from University of Padua. The author has contributed to research in topics: Leucine & GPX4. The author has an hindex of 30, co-authored 63 publications receiving 5042 citations. Previous affiliations of Giovanni Miotto include Pennsylvania State University & Penn State Milton S. Hershey Medical Center.

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Journal ArticleDOI

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Daniel J. Klionsky, +235 more
- 16 Feb 2008 - 
TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
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Transient and Long-Lasting Openings of the Mitochondrial Permeability Transition Pore Can Be Monitored Directly in Intact Cells by Changes in Mitochondrial Calcein Fluorescence

TL;DR: In hepatocytes and MH1C1 cells coloaded with Co2+ and calcein AM, treatment with MTP inducers caused a rapid, though limited, decrease in mitochondrial calce in fluorescence, which was significantly reduced by CsA, and MTP likely fluctuates rapidly between open and closed states in intact cells.
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Quantitation and Facilitated de Novo Sequencing of Proteins by Isotopic N-Terminal Labeling of Peptides with a Fragmentation-Directing Moiety

TL;DR: The approach is based on the use of an isotopically labeled reagent to quantitate (by mass spectrometry) the ratio of peptides from digests of a protein being expressed under different conditions, and allows quantitation of the changes occurring in spots or bands that contain more than one protein.
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Insight into the mechanism of ferroptosis inhibition by ferrostatin-1.

TL;DR: GPx4 and fer-1 in the presence of ferrous iron produces, by distinct mechanism, the most relevant anti-ferroptotic effect, i.e the disappearance of initiating lipid hydroperoxides.
Journal Article

Photosensitization with Zinc (II) Phthalocyanine as a Switch in the Decision between Apoptosis and Necrosis

TL;DR: The studies on the photosensitization of 4R transformed fibroblasts with the second-generation photosensitizer zinc (II) phthalocyanine with ZnPc demonstrate that it is possible to modulate the mechanism of cell death by appropriate protocols and propose possible correlations between the cell death mechanism and primary photo-damage sites.