Vickram Srinivas

TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.

TL;DR: It is shown that one isozyme, PFKFB3, is highly induced by hypoxia and thehypoxia mimics cobalt and desferrioxamine, and could be replicated by the use of an inhibitor of the prolyl hydroxylase enzymes responsible for the von Hippel Lindau (VHL)-dependent destabilization and tagging of HIF-1α.

TL;DR: The data suggest that MAPK signaling facilitates HIF activation through p300/CBP, and that the C-terminal transactivation domain of HIF-1α is not a direct substrate of MAPK, and Hif-1 α phosphorylation is not required for HIF/CAD/p300 interaction.

TL;DR: A new concept is presented: hypertrophic cells die through the induction of autophagy, which emphasizes the importance of the local microenvironment, in particular pO(2), in directing chondrocyte survival and apoptosis.

TL;DR: Analysis of the available cloned sequences of human and mouse members of the HIF-alpha family of proteins revealed an area of about 15 amino acids with strong sequence conservation between all the members, suggesting that the sequences and mechanisms involved in the regulated degradation of the alpha protein subunits are poorly understood.